中国药物警戒 ›› 2015, Vol. 12 ›› Issue (2): 79-81.

• 基于毒-效-证关联评价的山豆根毒性研究专栏 • 上一篇    下一篇

山豆根水提组分对实热证小鼠抗炎及伴随毒副作用研究

尹利顺1，2,吕莉莉1,窦立雯1，2,李晓宇1,孙蓉1*   

  1. 1山东省中医药研究院，山东 济南 250014;
    2山东中医药大学，山东 济南 250355
  • 收稿日期:2014-01-19 出版日期:2015-02-08 发布日期:2015-07-28
  • 通讯作者: 孙蓉，女，博士，研究员?博导，中药药理与毒理。
  • 作者简介:尹利顺，男，在读硕士，中药药理与毒理。
  • 基金资助:
    国家重点基础研究发展计划（973）中医基础理论专项资助项目（2009CB522802）。山东省自主创新和成果转化项目：药物安全性评价和适宜于抗肿瘤及缺血性脑血管疾病多靶点和复方新药成药性评价关键技术研究(2014ZZCX02104)。

Study on Anti-inflammatory Efficacy Accompanied by Toxicity and Side Effects of Water Extraction Components of Sophorae Tonkinensis Radix et Rhizoma on Throat Excess-heat Syndrome Mice

YIN Li-shun1,2，LV Li-li1，DOU Li-wen1,2，LI Xiao-yu1，SUN Rong1*   

  1. 1Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China;
    2Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2014-01-19 Online:2015-02-08 Published:2015-07-28

PDF (PC)

196

摘要： 目的 研究山豆根水提组分对咽喉实热证模型小鼠在抗炎作用下伴随出现的毒副作用，为其“功效-证候-毒性”相关性研究提供实验依据。方法 制备咽喉实热证小鼠模型，采用巴豆油致小鼠耳肿胀法，灌胃不同剂量山豆根水提组分，观察其抗炎及伴随毒副作用，检测血清中丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）活性和尿素氮（BUN）、肌酐（Cr）的含量，计算肝、肾、脾、胸腺脏体比值，评价其药效伴随毒副作用。结果 山豆根水提组分连续多次给药对巴豆油所致实热证小鼠耳肿胀具有较强的抑制作用，给药3天后血清ALT、AST活性明显升高，肝体比值增大。结论 药效剂量的山豆根水提组分对实热证小鼠具有较强的抗炎作用，同时会产生伴随毒副作用和肝毒性。与正常动物相比较，抗炎作用较强，肝毒性有所减弱，其在证候背景下毒副作用机制还有待进一步研究。

关键词: 山豆根, 实热证, 抗炎, 伴随毒副作用

Abstract: Objective To srudy the toxicity and side effects accompanied with anti-inflammation of water extraction components of Sophorae tonkinensis Radix et Rhizoma on throat excess-heat syndrome model in mice, provide experimental basis of "efficacy-syndrome-toxicity" relevancy. Methods The method of throat excess-heat syndrome model in mice was built, which were administrated with different doses water extraction components of Sophorae tonkinensis Radix et Rhizoma, observing anti-inflammation and toxicity by the croton oil inducing ear swelling method. detecting the level of ALT, AST, BUN, Cr in serum and calculating the ratio of liver and kidney to body, to evaluate its efficacy with side effects. Results Water extraction components of Sophorae tonkinensis Radix et Rhizoma had strong inhibitory effect on the croton oil inducing ear swelling in throat excess-heat syndrome mice after multiple administration. ALT and AST levels in serum were obviously enhanced after 3 d administration, liver weight/body ratio enhanced. Conclusion The effective dose of water extraction components of Sophorae tonkinensis Radix et Rhizoma had obvious anti-inflammatory effect, and at the same time, accompanied by side effects and hepatotoxicity. Compared with normal animals, anti-inflammatory effect is stronger and hepatotoxicity is weaker, whose toxicity mechanism still needs further research under the background of syndrome.

Key words: Sophorae tonkinensis Radix et Rhizoma, throat excess-heat syndrome, anti-inflammation, accompanied toxicity and side effects

中图分类号: