中国药物警戒 ›› 2022, Vol. 19 ›› Issue (10): 1073-1077.
DOI: 10.19803/j.1672-8629.20210203

• 基础与临床研究 • 上一篇    下一篇

芪黄益气摄血方对免疫性血小板减少症模型小鼠的治疗作用及抗炎机制研究

罗雅琴1, 蔡强2, 黄伟3,4,*   

  1. 1山东中医药大学附属医院,山东 济南 250014;
    2山东电力中心医院,山东 济南 250001;
    3山东省中医药研究院,山东 济南 250014;
    4齐鲁工业大学,山东省科学院,山东省分析测试中心,山东 济南 250014
  • 收稿日期:2021-03-10 出版日期:2022-10-15 发布日期:2022-10-17
  • 通讯作者: *黄伟,男,博士,副研究员,中药药效物质基础及作用机理研究。E-mail:huangwei 986@126.com
  • 作者简介:罗雅琴,女,硕士,主治医师,中医药治疗血液病的基础与临床研究。
  • 基金资助:
    国家自然科学基金资助项目（81704029）; 齐鲁卫生与健康杰出青年人才资助项目（鲁卫人才字[2020] 3号）; 中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”(2060302); 山东省中医药科技面上项目（2020M027）; 全国名老中医药专家刘持年传承工作室建设项目（国中医药人教发[2014]20号）

Therapeutic effect and anti-inflammatory mechanism of Qihuang Yiqi Shexue Formula in ITP model mice

LUO Yaqin1, CAI Qiang2, HUANG Wei3,4,*   

  1. 1The Affiliated Hospital of Shandong University of Chinese Medicine, Jinan Shandong 250014, China;
    2Shandong Electric Power Central Hospital, Jinan Shandong 250001, China;
    3Shandong Academy of Chinese Medicine, Jinan Shandong 250014 China;
    4Qilu University of Technology, Shandong Academy of Sciences, Shandong Analysis and Test Center, Jinan Shandong 250014, China
  • Received:2021-03-10 Online:2022-10-15 Published:2022-10-17

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摘要： 目的 探讨芪黄益气摄血方对免疫性血小板减少症（ITP）模型小鼠的治疗作用及抗炎机制。方法 50只BALB/c小鼠随机分为正常组,模型组,芪黄益气摄血方高、低剂量组和强的松组,每组10只。采用豚鼠抗小鼠血小板血清（APS）腹腔注射方法,建立ITP模型;注射APS后的第8天开始给药,连续灌胃14 d。观察小鼠一般情况;检测外周血血小板计数（PLT）和血红蛋白（Hb）浓度变化;Elisa法检测血清血小板生成素（TPO）、促炎因子白介素-2（IL-2）、IL-6、肿瘤坏死因子-α（TNF-α）、干扰素-γ（IFN-γ）及抑炎因子IL-4、IL-10、转化生长因子-β1（TGF-β1）水平;分离脾脏、胸腺,称重,计算脏器指数;取胸骨做骨髓涂片,显微镜下进行骨髓巨核细胞分类。结果 与正常组比较,模型组小鼠PLT、Hb、TPO、IL-4、IL-10、TGF-β1显著降低（P<0.05或P<0.01）,骨髓产板巨核细胞数明显减少（P<0.01）,血清IL-2、IL-6、TNF-α、IFN-γ以及脾脏和胸腺指数显著升高（P<0.05或P<0.01）;与模型组比较,各给药组PLT、TPO、IL-4、IL-10、TGF-β1水平明显升高（P<0.05或P<0.01）,骨髓产板巨核细胞数明显增加（P<0.01）,血清IL-2、IL-6、TNF-α、IFN-γ以及脾脏和胸腺指数显著降低（P<0.05或P<0.01）,仅高剂量组Hb显著增加（P<0.05）;与低剂量组比较,高剂量和强的松组PLT、IL-4、TGF-β1显著升高（P<0.05或P<0.01）,TNF-α显著降低（P<0.05或P<0.01）,仅强的松组IL-6显著降低（P<0.05）,高剂量组IL-10显著升高（P<0.05）,其余指标无统计学差异（P>0.05）。结论 芪黄益气摄血方可通过调节ITP模型小鼠促炎因子和抑炎因子的分泌失衡,增加骨髓产板巨核细胞和TPO水平,发挥治疗ITP的作用。

关键词: 免疫性血小板减少症, 芪黄益气摄血方, 治疗作用, 抗炎机制

Abstract: Objective To explore the therapeutic effect and anti-inflammatory mechanism of Qihuang Yiqi Shexue Formula in immune thrombocytopenia (ITP) model mice. Methods Fifty BALB/c mice were randomly divided into the normal group, model group, high and low dose groups of Qihuang Yiqi Shexue Formula, and the prednisone group, with 10 mice in each. The intraperitoneal injection of guinea pig anti-mouse platelet serum (APS) was used to establish an ITP mouse model. On the 8th day after the injection of APS, each group was given intragastric administration for 14 consecutive days. The general condition of each group of mice was observed, changes in peripheral blood platelet count (PLT) and hemoglobin (Hb) concentrations were detected, and the Elisa method was used to detect serum levels of thrombopoietin (TPO), pro-inflammatory factor interleukin-2 (IL-2), IL-6, tumor necrosis factor-α(TNF-α), interferon-γ(IFN-γ), anti-inflammatory factor IL-4, IL-10, and transforming growth factor-β1 (TGF-β1). The spleen and thymus tissues were separated, weighed, and the organ index was calculated. The sternum was taken as a bone marrow smear, and the bone marrow megakaryocytes were classified under a microscope. Results Compared with the normal group, the levels of PLT, Hb, TPO, IL-4, IL-10 and TGF-β1 in the model group significantly decreased (P<0.05 or P<0.01), the number of megakaryocytes in the bone marrow plate became significantly smaller (P<0.01), and serum levels of IL-2, IL -6, TNF-α and IFN-γ were significantly decreased (P<0.05 or P<0.01). Compared with the model group, the levels of PLT, TPO, IL-4, IL-10, and TGF-β1in each administration group were significantly increased (P<0.05 or P<0.01), so was the number of megakaryocytes in the bone marrow plate (P<0.01) , but serum levels of IL-2, IL-6, TNF-α and IFN-γ were significantly decreased (P<0.05 or P<0.01). Compared with the low-dose group, the levels of PLT, IL-4 and TGF-β1 were significantly increased in the high dose group and the prednisone group, but TNF-α was significantly decreased (P <0.05 or P <0.01). There was no statistically significant difference in other indicators (P>0.05). Conclusion Qihuang Yiqi Shexue Formula is effective for ITP by regulating the unbalanced secretion of pro-inflammatory factors and anti-inflammatory factors in ITP model mice, increasing the levels of bone marrow-producing megakaryocytes and TPO.

Key words: immune thrombocytopenia (ITP), Qihuang Yiqi Shexue Formula, therapeutic effect, anti-inflammatory mechanism

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