中国药物警戒 ›› 2014, Vol. 11 ›› Issue (5): 271-274.

• 质量与工艺研究 • 上一篇    下一篇

HPLC法测定附子及其样品中6种乌头类生物碱的含量

栾永福1, 孙蓉2*   

  1. 1天津中医药大学,天津 300073;
    2山东省中医药研究院,山东 济南 250014
  • 收稿日期:2016-02-03 修回日期:2016-02-03 出版日期:2014-05-08 发布日期:2016-03-02
  • 作者简介:栾永福,男,在读硕士,中药药理学。
  • 基金资助:
    国家重点基础研究发展计划 （973）中医基础理论专项资助项目课题2（2009CB522802）

Determination of Six Kinds of Aconitum Alkaloids in Aconitum Carmichaeli and Its Samples by HPLC

LUAN Yong-fu1, SUN Rong2, *   

  1. 1Tianjin University of Traditional Chinese Medicine, Tianjin 300073, China;
    2Shandong Research Academy of TCM, Shandong Ji'nan 250014, China
  • Received:2016-02-03 Revised:2016-02-03 Online:2014-05-08 Published:2016-03-02

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摘要： 目的建立同时测定附子药材及其样品中6种乌头类生物碱含量的高效液相色谱分析方法。方法C18柱,乙腈-四氢呋喃（25：15）为流动相A,以0.1mol·L-1醋酸铵溶液为流动相B,梯度洗脱,流速1.0mL·min-1,检测波长235nm。结果6种乌头类生物碱均达到了较好的分离,分别在一定范围内呈良好的线形关系。结论该方法简便、快速、灵敏,可有效地用于附子的鉴别和内在质量控制。

关键词: 附子, 乌头类生物碱, 高效液相色谱法

Abstract: ObjectiveTo establish a HPLC method for the determination of six kinds of Aconitum alkaloids in Aconitum carmichaeli and its samples. MethodsA C18 column was used, and acetonitrile- tetrahydrofuran(25:15) as the mobile phase A, 0.1mol·L-1 NH4AC solution as the mobile phase B, gradient elute with a flow rate of 1.0mL·min-1 and a detection wavelength of 235nm. ResultsThe six kinds of Aconitum alkaloids were separated, and the calibration curves of them were in good linearity over the certain range respectively. ConclusionThis method is simple and accurate. It can be used in the identification and quality control of A. carmichaeli.

Key words: Aconitum carmichaeli, Aconitum alkaloid, HPLC

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