中国药物警戒 ›› 2022, Vol. 19 ›› Issue (1): 69-71.
DOI: 10.19803/j.1672-8629.2022.01.14

• 基础与临床研究 • 上一篇    下一篇

反相高效液相色谱法测定骨髓感染引流液中克林霉素的浓度

王培旭1, 徐玲笑2, 刘淑花1,*   

  1. 1烟台市烟台山医院,山东 烟台 264000;
    2衢州市食品药品检验所,浙江 衢州 324002
  • 收稿日期:2021-08-09 出版日期:2022-01-15 发布日期:2022-01-20
  • 通讯作者: *刘淑花,女,主任药师,药物制剂及药事管理。E-mail：liushuhua1965@163.com
  • 作者简介:王培旭,男,主管药师,药物治疗研究。
  • 基金资助:
    国家科技重大专项（2015ZX09501004-001-001）

Determination of clindamycin in drainage fluid of bone marrow infection by HPLC

WANG Peixu1, XU Lingxiao2, LIU Shuhua1,*   

  1. 1Yantaishan Hospital, Yantai Shandong 264000, China;
    2Quzhou City Food and Drug Inspection Institute, Quzhou Zhejiang 324002, China
  • Received:2021-08-09 Online:2022-01-15 Published:2022-01-20

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摘要： 目的 建立测定引流液中克林霉素含量的方法,探求骨髓感染抗菌药物载药系统克林霉素+半水硫酸钙骨水泥临床应用意义。方法 采用Agilent ZORBAX Extend-C18（150 mm×4.6 mm,5 µm）为色谱柱;磷酸盐缓冲液（每1 mL中含磷酸二氢钾6.8 mg,用25 %的氢氧化钾调节pH至7.5）：乙腈=55∶45为流动相;检测波长为210 nm;流速为1.0 mL·min-1。结果 克林霉素在1.48~88.90 μg·mL-1范围内呈良好线性关系（r=0.999 7）,低、中、高浓度的平均回收率为92.90%、98.03%、97.13%;日内和日间相对标准偏差（RSD）分别小于2.0%和3.5%。结论 反相高效液相色谱法测定引流液中克林霉素的浓度方法简便、灵敏、准确,对载克林霉素半水硫酸钙骨水泥临床应用有一定参考意义。

关键词: 高效液相色谱法, 骨髓感染, 引流液, 克林霉素, 半水硫酸钙

Abstract: Objective To establish an HPLC method for determining clindamycin in drainage fluid and explore the clinical applicability of clindamycin hemihydrate calcium sulfate bone cement in the local antibacterial drug delivery system. Methods Agilent ZORBAX Extend-C18 (150 mm×4.6 mm, 5 µm) was used as the column. Phosphate buffer (6.8 mg·mL-1 KH2PO4, pH adjusted to 7.5 with 25% KOH) and acetonitrile (55∶45) served as the mobile phase. The detection wavelength was 210 nm and the flow rate was 1.0 mL·min-1. Results Clindamycin presented a good linear range of 1.48 to 88.90 μg·mL-1 (r=0.999 7). The average recoveries were 92.90%, 98.03% and 97.13% respectively. The RSD values were less than 2.0% and 3.5%. Conclusion The reversed-phase high performance liquid chromatography method for the determination of concentrations of clindamycin in drainage fluid is simple, sensitive and accurate, which is of referential value for clinical applications of clindamycin semi-water calcium sulfate bone cement.

Key words: HPLC, bone marrow infection, drainage fluid, clindamycin, semi-water calcium sulfate

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