中国药物警戒 ›› 2014, Vol. 11 ›› Issue (3): 158-159.

• 质量与工艺研究 • 上一篇    下一篇

黛蛤散中有效成分靛玉红的含量测定

刘峰   

  1. 日照市药品检验所,山东 日照 276800
  • 收稿日期:2013-08-15 修回日期:2016-02-03 出版日期:2014-03-08 发布日期:2016-02-03
  • 作者简介:刘峰,男,副主任医师,药品检验。

Determination of Indirubin in Daige Powders

LIU Feng   

  1. Institute for Drug Control of Rizhao，Shandong Rizhao 276800, China
  • Received:2013-08-15 Revised:2016-02-03 Online:2014-03-08 Published:2016-02-03

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摘要： 目的建立黛蛤散中靛玉红含量的HPLC测定方法。方法采用Hypersil C18柱（4.6 mm×250 mm,5μm）为色谱柱,流动相为甲醇-水（70:30）,检测波长为292nm,柱温为30℃,流速为1 mL·min-1。结果靛玉红的进样量在0.01~0.20μg范围内线性关系良好,（r =0.9999）,平均回收率为98.5 %,（RSD= 1.00%）。结论本方法可用于测定黛蛤散中靛玉红的含量,结果准确,重复性好。

关键词: 黛蛤散, 靛玉红, 含量测定

Abstract: Objective To develop a HPLC method for determination of indirubin in Daige powders. Methods The optimum HPLC conditions were as follows Hypersil C18 column(4.6 mm× 250 mm,5μm), methanol-water(7030)as mobile phase, detection wavelength 292nm, column temperature 30℃, flow rate 1mL·min-1. Results There was good linearity between peak areas and injection quantity of indirubin within the range of 0.01~0.20μg(r=0.9999).The average recovery was 98.5% for indirubin(RSD=1.00%). Conclusion The method can detect indirubin in Daige powders. The HPLC method is reasonable, stable and accurate.

Key words: Daige powders, indirubin, determination of content