中国药物警戒 ›› 2014, Vol. 11 ›› Issue (12): 705-708.

• 基础与临床研究 •    下一篇

柴胡皂苷d对小鼠急性毒性实验研究

李晓宇1, 窦立雯2, 孙蓉1 *   

  1. 1山东省中医药研究院,山东 济南 250014;
    2山东中医药大学,山东 济南 250014
  • 收稿日期:2014-10-27 修回日期:2016-02-03 出版日期:2014-12-08 发布日期:2016-03-02
  • 通讯作者: 孙蓉,女,博士,研究员·博导,中药药理与毒理研究。E-mail：sunrong107@163.com
  • 作者简介:李晓宇,女,硕士,中药药理与毒理。
  • 基金资助:
    山东省自然科学基金重点课题（2011ZRB14333）,山东省科技平台建设项目课题（2008GG2NS02021）,国家自然科学基金课题（81073148,30672649,81374059）

Experimental Study on Mice's Acute Toxicity of Saikosaponin d

LI Xiao-yu1, DOU Li-wen2, SUN Rong1, *   

  1. 1Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China; 2Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2014-10-27 Revised:2016-02-03 Online:2014-12-08 Published:2016-03-02

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摘要： 目的探讨柴胡皂苷d对小鼠急性毒性的影响。方法采用经典小鼠急性毒性实验方法,进行柴胡皂苷d对小鼠灌胃给药最大给药量和腹腔给药半数致死量的急性毒性研究,均连续观察14天。结果按柴胡皂苷d重量计算,柴胡皂苷 d 灌胃给药的最大给药量为770.47 mg·kg-1·d-1,腹腔给药小鼠的半数致死量为62.338 mg·kg-1·d-1,95%可信限为53.703~70.966 mg·kg-1·d-1。柴胡皂苷d 对小鼠灌胃给药,小鼠急性毒性主要表现为安静、伏卧不动,即而出现呼吸急促、眼睑下垂、毛发乍起现象,但未见死亡。柴胡皂苷d 对小鼠腹腔注射给药,死亡小鼠急性毒性主要表现为安静怠动,即而出现走路不稳、呼吸急促、眼睑下垂、毛发乍起、间歇性抽搐、神经抑制而死亡。存活小鼠在14天观察期内体重增长缓慢,与空白对照组比较有明显差异。死亡小鼠解剖观察,有肝脏病变,余未见明显异常。结论腹腔注射柴胡皂苷d的小鼠急性毒性大,毒性反应强烈,证实了柴胡皂苷d 是柴胡总皂苷和柴胡产生毒性的主要化学成分之一。其体内蓄积毒性的“量-时-毒”关系以及在体内、外的生物转化过程,毒性作用部位、机制有待进一步研究。

关键词: 柴胡皂苷d, 小鼠, 急性毒性, 半数致死量

Abstract: ObjectiveTo discuss the effect of saikosaponin d on mice's acute toxicity. MethodsMice's acute toxicities of different drug-delivery ways were tested respectively by the classic methods of acute toxicity. ResultsCaculated by saikosaponind, the MLD of ig is 770.47 mg·kg-1·d-1, the LD50 of ip is 62.338 mg·kg-1·d-1, and the 95% confidence interval is 53.703~70.966 mg·kg-1·d-1. The main acute toxicity symptoms of mice are negligent action, then unsteady gait, shortness of breath,drooping eyelids,hair rising, intermittent convulsion, neurological inhibition by ig.The signs of dead mice are neurological inhibition, and eventually to death by ip. The surviving mice's weight gainings are slower in 14 d observation period and compared with the control group, there is no signifi cant difference. By anatomical observation of death mice, there are no other obvious abnormalities except hepatic pathological changes.ConclusionThe acute toxicity of saikosaponin d is high, and we further confirmed that saikosaponin d is one of mainly toxical material composition of bupleurum.The "dose-timepoison"relationships of accumulated toxicity, biotransformation process in vivo and in vitro, and the position and mechanism of toxic effects are to be further studied.

Key words: saikosaponin d, mice, acute toxicity, LD50

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