中国药物警戒 ›› 2014, Vol. 11 ›› Issue (12): 709-713.

• 基础与临床研究 • 上一篇    下一篇

银丹心脑通软胶囊对血管内皮细胞的保护作用

王岚1, 殷小杰1, 杨洪军1, 王建露1, 许海玉1, 贡磊磊1, 原会俊2, 梁日欣1 *   

  1. 1中国中医科学院中药研究所,北京 100700;
    2首都医科大学中医药学院,北京 100069
  • 收稿日期:2014-10-27 修回日期:2016-02-03 出版日期:2014-12-08 发布日期:2016-03-02
  • 通讯作者: 梁日欣,女,研究员,博导,中药药理学。
  • 作者简介:王岚,女,副研究员,中药药理学。
  • 基金资助:
    国家重大新药创制科技重大专项（2012ZX09201201）

Protective Effect of Yindan Xinnaotong Capsule on Endothelial Cells in Vitro

WANG Lan1, YIN Xiao-jie1, YANG Hong-jun1, WANG Jian-lu1, XU Hai-yu1, GONG Lei-lei1, YUAN Hui-jun   

  1. 1Institute of Chinese Material Medical, China Academy of Chinese Medical Sciences, Beijing 100700, China; 2School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China
  • Received:2014-10-27 Revised:2016-02-03 Online:2014-12-08 Published:2016-03-02

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摘要： 目的研究银丹心脑通软胶囊（YXC）对血管内皮细胞的保护作用及作用机制。方法采用氧化低密度脂蛋白（Ox-LDL）和肿瘤坏死因子α（TNF-α）诱导人脐静脉内皮细胞（HUVEC）损伤两种模型,MTT法测定YXC预处理后HUVEC存活率,探讨YXC对内皮细胞的保护作用;采用流式细胞术测定YXC对内皮细胞凋亡的影响,探讨YXC内皮保护的作用机制。结果YXC的最大无毒浓度为500 μg·mL-1。将250、125、62.5 μg·mL-1 3个浓度作为YXC的实验浓度;YXC孵育 24 h后可明显升高Ox-LDL诱导的HUVEC的存活率（P <0.05）,具有明显的量效关系; YXC孵育 24 h后可明显升高TNF-α诱导的HUVEC的存活率（P <0.05）。250和125 μg·mL-1 YXC 可显著降低HUVEC细胞的凋亡率。结论YXC对血管内皮细胞具有保护作用,其机制可能与抗细胞凋亡有关。

关键词: 银丹心脑通软胶囊, 血管内皮细胞, 细胞损伤, 细胞凋亡

Abstract: ObjectiveTo study the protective effect of Yindan Xinnaotong capsule(YXC) on endothelial cells. MethodsHuman umbilical vein endothelial cells (HUVECs) were cultured in vitro and HUVECs were pretreated with 250 μg·mL-1,125 μg·mL-1 and 62.5 μg·mL-1 for 24 h. 200 μg·mL-1 Ox-LDL and 60 ng·mL-1 TNF-α were used to induce HUVECs injury. The cell survival rate was determined by MTT, and flow cytometry was used to detect apoptosis to evaluate the mechanism of protective function of YXC. ResultsThe maximal non-cytotoxic concentration of YXC was 500 μg·mL-1, thus 250, 125 and 62.5 μg·mL-1 were employed as the experimental concentrations. YXC could obviously increase the survival rate of HUVECs induced by Ox-LDL (P <0.05) and TNF-α with a dose-dependence manner; compared with model group, 62.5 and 250 μg·mL-1 YXC treatment decreased apoptosis rate induced by Ox-LDL HUVECs (P <0.05). Meanwhile, 250 and 125 μg·mL-1 YXC obviously reduced apoptosis rate induced by TNF-α (P <0.05). ConclusionYXC could protect the HUVEC injury induced by Ox-LDL and TNF-α, and this may be associated with the inhibitory effect on cell apoptosis.

Key words: Yindan Xinnaotong capsule, endothelial cells, cell damage, cell apoptosis

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