中国药物警戒 ›› 2014, Vol. 11 ›› Issue (1): 4-7.

• 基础研究 • 上一篇    下一篇

SD大鼠被动皮肤过敏实验影响因素的研究

戴学栋1,2,方超1戴晓莉1,马玉奎1   

  1. 1山东省医药工业研究所,山东 济南,250033;
    2山东省化学药物重点实验室,山东 济南,250033
  • 收稿日期:2016-02-03 修回日期:2016-02-03 出版日期:2014-01-08 发布日期:2016-02-03

Study on Experimental Factors of Passive Cutaneous Anaphylaxis in SD Rats

DAI Xue-dong1, 2, FANG Chao1,DAI Xiao-li1, MA Yu-kui1   

  1. 1Shandong Institute of Pharmaceutical Industry, Shandong Jinan 250033, China;
    2Shandong Provincial Key Laboratory of Chemical Drug, Shandong Jinan 250033, China
  • Received:2016-02-03 Revised:2016-02-03 Online:2014-01-08 Published:2016-02-03

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摘要： 目的探讨SD大鼠被动皮肤过敏反应（PCA）的影响因素,为选择敏感实验条件提高实验准确性提供依据。方法采用SD大鼠作为实验动物,在不同的实验条件下（加或不加佐剂、抗原种类、致敏剂量、致敏途径、致敏次数）进行PCA实验。结果采用卵白蛋白（OVA）作为抗原,SD大鼠在不加佐剂时,PCA反应为阴性;在加入佐剂时PCA反应均为阳性,蓝斑直径及阳性反应率从大到小依次为：氢氧化铝凝胶>百白破疫苗>弗氏完全佐剂。采用氢氧化铝凝胶作为佐剂时,OVA对SD大鼠的免疫原性优于牛血清白蛋白（BSA）。增加致敏剂量能使大鼠背部蓝斑直径明显增大。腹腔致敏和背部皮下致敏均可良好的PCA反应,两者之间无明显差异性。增加致敏次数对PCA反应无明显影响。结论采用SD大鼠进行PCA实验最佳实验条件为：采用OVA作为抗原,氢氧化铝凝胶作为佐剂,两者按照1:1混合后,皮下隔日致敏,OVA浓度为10mg·mL-1,致敏体积为1mL,共致敏3次,末次致敏后10天腹主动脉取血,分离抗血清,皮下被动致敏,24h后以相同量抗原静脉激发。

关键词: SD大鼠, 被动皮肤过敏, 佐剂

Abstract: Objective To gain sensitive experimental conditions and improve the accuracy of passive cutaneous anaphylaxis(PCA) test through investigating the influencing factors of PCA in SD rats. MethodsHomologous PCA was performed by using SD rats, and were carried out in different experimental conditions(with or without adjuvant, OVA or BSA was taken as allergen, sensitizing doses, sensitizing means, sensitizing times). ResultsHomologous PCA revealed negative reaction without adjuvant, showed positive reaction with adjuvant. According to diameter of dermal Evan's blue spot and the positive reaction rate, the degree of response was as follows: Al(OH)3>DPT vaccine>CFA. The immunogenicity of OVA was superior to BSA in SD rats when Al(OH)3 was added as adjuvant in PCA test. The diameter of dermal Evan's blue spot accreted by increasing sensitizing dose. Intraperitoneal and subcutaneous injection sensitization both could get good response of PCA, and there were no significant deviations between two sensitizing means. There was no obvious impact on PCA response by increasing sensitizing times. ConclusionThe optimal conditions in homologous PCA in SD rats are as follows: SD rats are sensitized with OVA 10mg/rat and Al(OH)3 which is added as adjuvant through subcutaneous injection every other day, three times in all. Blood is gained from abdominal aorta on the tenth day after the last sensitization, and blood serum is gained by centrifugalization. The other SD rats are sensitized with 1mL blood serum by endermic injection, and then, SD rats are stimulated with OVA 10mg/rat with equal volume 5% Evans blue.

Key words: SD rat, PCA, adjuvant

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