中国药物警戒 ›› 2013, Vol. 10 ›› Issue (10): 614-615.

• 药品质量与工艺研究 • 上一篇    下一篇

HPLC法测定脂降宁片中大黄素的含量

耿海霞1,王洪明2*   

  1. 1.滨州市食品药品监督管理局滨城区分局,山东滨州 256600；
    2.滨州市药品检验所,山东滨州 256618
  • 收稿日期:2013-07-15 修回日期:2016-03-09 出版日期:2013-10-08 发布日期:2016-03-09
  • 通讯作者: 王洪明,本科,主管药师,药品检验及药物质量研究。 E-mail：86884533@qq.com
  • 作者简介:耿海霞,女,药师,药品检验及质量监督管理。

Determination of Emodin in Zhijiangning Tablet by HPLC

GENG Hai -xia1, WANG Hong -ming2*   

  1. 1.Bincheng Food and Drug Administration, Shandong Binzhou 256600, China;
    2.Binzhou Institute for Drug Control, Shandong Binzhou 256618, China
  • Received:2013-07-15 Revised:2016-03-09 Online:2013-10-08 Published:2016-03-09

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摘要： 目的 建立脂降宁片中大黄素含量的高效液相色谱测定方法。方法 AgilentExtendC18色谱柱（250×4.6mm,5μm）,以甲醇-0.1%磷酸溶液（80：20）为流动相,检测波长为290nm。结果 大黄素进样量在0.005025μg～0.1005μg的范围内呈良好的线性关系（r=0.9999）,平均回收率为99.2%。结论 本方法简单,准确,重复性好,可用于该药品的质量控制。

关键词: HPLC, 脂降宁片, 大黄素, 含量测定

Abstract: Objective To establisha RP -HPLC method for the determination of emodin in Zhijiangning tablet. Methods Agilent Extend C18 column was used. The mobile phase was methanol -0.1% phosphoric acid (80:20). The detection wavelength was set at 290nm. Results A good linearity was obtained in the range of 0.005025μg to 0.1005μg(r=0.9999).The average recovery was 99.2%. Conclusion The method is simple,accurate and repeatable and can be used for the quality control.

Key words: HPLC, Zhijiangning tablet, emodin, determination

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