中国药物警戒 ›› 2012, Vol. 9 ›› Issue (7): 387-391.

• 基于“功效”的有毒中药毒性综合评价 • 上一篇    下一篇

鸦胆子不同组分发挥抗炎作用的安全范围研究

郑世存1, 栾永福2, 罗栋3, 朱兰兰2, 谢元璋4, 孙蓉1*   

  1. 1山东省中医药研究院,山东 济南 250014；
    2天津中医药大学,天津 300193；
    3 山东中医药大学,山东 济南 250355；
    4 哈尔滨商业大学,黑龙江 哈尔滨 150076
  • 收稿日期:2012-05-15 出版日期:2012-07-10 发布日期:2015-08-07
  • 通讯作者: 孙蓉,女,研究员 ·博导,博士,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:郑世存,男,副主任医师,临床医学与卫生管理。
  • 基金资助:
    国家重点基础研究发展计划 （973）中医基础理论专项资助项目课题2（2009CB522802）

The Margin of Safety Study on Anti-inflammatory Effect of Different Components from Fructus Bruceae

ZHENG Shi-cun1, LUAN Yong-fu2, LUO Dong3, ZHU Lan-lan2, XIE Yuan-zhang4, SUN Rong1*   

  1. 1 Shandong Research Academy of TCM, Jinan 250014, China;
    2Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
    3 Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 4Harbin University of Commerce, Heilongjiang Harbin 150076, China
  • Received:2012-05-15 Online:2012-07-10 Published:2015-08-07

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摘要： 目的研究鸦胆子不同组分发挥抗炎作用的治疗指数和安全范围。方法通过建立巴豆致小鼠耳肿胀模型和琼脂致小鼠肉芽肿胀模型探究鸦胆子不同组分抗急、慢性炎症作用,并进行鸦胆子不同组分急性致死量实验。计算各组分发挥抗炎药效的ED50和急性毒性试验的LD50和LD5,求得不同组分发挥抗炎作用的治疗指数（TI）,安全系数（SF）。结果鸦胆子不同组分对炎症模型有明显的抗炎作用,并呈现一定的量效关系,且水提组分抗炎作用大于醇提组分。鸦胆子水提、醇提组分对耳肿胀模型小鼠的抗炎TI、SF分别为35.6207、7.8040和25.3285、3.6645;鸦胆子水提、醇提组分对肉芽肿模型小鼠的抗炎TI、SF分别为47.6659、7.5494和32.6431、4.2959。结论鸦胆子醇提组分发挥抗炎作用的安全范围大于水提组分,在应用鸦胆子水提组分时更应密切注意不良反应的发生并加强用药监测。

关键词: 鸦胆子, 抗炎, 治疗指数, 安全系数

Abstract: Objective To research therapeutic index(TI) and the margin of safety factor(SF) of anti-inflammatory effect of Fructus Bruceae. Methods Establish tumidness model induced by daubing croton oil and granuloma model induced by subcutaneous injection of agar to explore acute and chronic anti-inflammatory effect of different components from Fructus Bruceae, and acute lethal dose test is also proceeded. Calculate ED50 and ED95 of anti-inflammatory efficacy and LD50 and LD5 of acute toxicity test of the different components from Fructus Bruceae. And then calculate the therapeutic index(TI) and safety factor(SF) of different components from Fructus Bruceae. Results The different components from Fructus Bruceae showed good anti-inflammatory effect and certain dose-effect relationship. The anti-inflammatory effect of alcohol extraction component was better than it of water extraction component. TI of anti-acute inflammatory effect of the water extraction component and the alcohol extraction component from Fructus Bruceae is 35.6207 and 25.3285, and SF is 7.8040 and 3.6645 respectively. And TI of anti-chronic inflammatory effect of the water extracts and the alcohol extracts from Fructus Bruceae is 47.6659 and 32.6431, and SF is 7.5494 and 4.2959 respectively. Conclusion The anti-inflammatory effect of the water extracts is greater than the alcohol extracts from Fructus Bruceae, but the margin of safety from alcohol extracts is greater than the water extracts from Fructus Bruceae. We should pay more attention to the occurrence of adverse reactions and strengthen drug monitoringin in the application of the water extracts from Fructus Bruceae.

Key words: Fructus Bruceae, anti-inflammatory, the margin of safety, therapeutic index, safety factor