中国药物警戒 ›› 2012, Vol. 9 ›› Issue (4): 205-206.

• 基础及临床研究 • 上一篇    下一篇

高效液相色谱法测定姜黄颗粒中姜黄素成分含量

周颖1，刘扬2，成旭东2，*   

  1. 1 苏州市第四人民医院药剂科，江苏 苏州 215001；
    2 苏州大学药学院，江苏 苏州 215123
  • 收稿日期:2012-02-27 出版日期:2012-04-10 发布日期:2015-08-10
  • 通讯作者: 成旭东,男,工程师,硕士,中药新药研发及质量控制。E-mail：chengxudong@yahoo.cn
  • 作者简介:周颖，女，主管药师，药物质量控制。

Determination of Curcumin in Jianghuang Granules by HPLC

ZHOU Ying1, LIU Yang2, CHENG Xu-dong2, *   

  1. 1 Suzhou No.4 People's Hospital, Jiangsu Suzhou 215001, China;
    2 College of Pharmaceutical Science, Soochow University, Jiangsu Suzhou 215123, China
  • Received:2012-02-27 Online:2012-04-10 Published:2015-08-10

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摘要： 目的建立姜黄素高效液相测定方法，测定姜黄颗粒中姜黄素成分含量;方法 采用RP-HPLC，色谱柱Shimpack VP-ODS（4.6mm*150mm，5μm）;流动相：乙腈-4%冰醋酸（48: 52）;检测波长：430nm;流速：1mL·min-1;柱温30℃。结果姜黄素的进样浓度在2.104~105.2μg·mL-1范围内与峰面积积分值呈良好线性关系（r=0.9998）;平均加样回收率为99.68％，RSD=1.34％（n=9）;结论 该法稳定、简便，可用于姜黄颗粒的质量控制。

关键词: 姜黄颗粒, 姜黄素, 高效液相色谱法, 含量测定

Abstract: Objcetive To establish the method for the content determination of curcumin in Jianghuang Granules. Methods HPLC method was adopted. Chromatographic conditions were as follows: Shimpack VP-ODS(150 mm×4.6 mm, 5μm) column was used with mobile phase consisted of acetonitrile-4％ phosphoric acid(48∶52) at a flow rate of 1.0 mL·min-1 under a detection wavelength at 430nm. The column temperature was 30℃. Results The linear range of Curcumin was 2.104~105.2 μg·mL-1(r=0.999 8) with an average recovery rate of 99.68％(RSD=1.34％, n=9). Conclusion The method is simple, reliable and accurate, and can be used for the quality control of Jianghuang Granules.

Key words: Jianghuang Granules, curcumin, HPLC, content determination