中国药物警戒 ›› 2011, Vol. 8 ›› Issue (7): 407-409.

• 艾叶毒性与毒靶器官研究 • 上一篇    下一篇

艾叶不同组分致小鼠肝毒性氧化损伤机制研究

龚彦胜1, 张亚囡2, 黄伟2, 王会2, 孙蓉1, *   

  1. 1.山东省中医药研究院,山东 济南 250014;
    2.山东中医药大学,山东 济南 250355
  • 收稿日期:2010-10-08 出版日期:2011-07-10 发布日期:2015-07-13
  • 通讯作者: *通讯作者：孙蓉,女,研究员,硕士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:龚彦胜,男,助理研究员,中药药理与毒理。
  • 基金资助:
    国家重点基础研究发展计划 （973）中医基础理论专项资助项目 （2009CB522802）,山东省科技平台建设项目课题（2008GG2NS02021）

The Research of Oxidative Damage Mechanism in Hepatical Toxical Injury Caused by Different Components from Folium Artemisiae Argyi in Mice

GONG Yan-sheng1, ZHANG Ya-nan2, HUANG Wei2, WANG Hui2, SUN Rong1, *   

  1. 1.Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2.Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China
  • Received:2010-10-08 Online:2011-07-10 Published:2015-07-13

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摘要： 目的 观察连续给予艾叶水提组分和挥发油组分导致小鼠肝毒性损伤程度与氧化损伤的相关性。方法 小鼠按照艾叶水提组分高、中、低剂量分别为9.0,4.68,1.17g·kg-1,挥发油组分高、中、低剂量组分别为1.25,0.50,0.13 mL·kg-1连续给药7天,检测血和肝组织内MDA含量、SOD活性、一氧化氮（NO）、一氧化氮合酶（NOS）、谷胱甘肽（GSH）和谷胱甘肽过氧化物酶（GSH-Px）的含量和活性。结果 艾叶水提组分和挥发油组分可致血中和肝组织内MDA含量增加,同时SOD活性下降;血和肝组织中NO含量增加,NOS活性升高;血和肝组织中GSH含量下降,GSH-Px活性下降。上述变化趋势随剂量增加而加重,与空白对照组有明显差异。结论 艾叶水提组分和挥发油组分多次给药后可导致小鼠肝毒性损伤,其损伤途径与引起机体氧化应激后诱导脂质过氧化有关。

关键词: 艾叶, 不同组分, 肝毒性, 氧化损伤

Abstract: Objective To investigate the relationship of oxidative damage mechanism in hepatical toxical injury caused by different components from Folium Artemisiae Argyi in mice. Methods Mice were administrated with water extracted and volatile oil components according to 7 days toxicity test method. The level of MDA、SOD、NO、NOS、GSH、GSH-Px in serum and hepatic tissue were detected. Results The different components can cause the activity of MDA in serum and liver increased, the level of SOD corresponding induced, however the level of NO and NOS were increased. In addition the activity of GSH and GSH-Px reduced. All the changes were aggravated in accordance with the dosages. Conclusion The continuous administration of different components from Folium Artemisiae Argyi to mice can induce obvious hepato-toxicity injury, the approach of hepatic damage is related with the peroxidative damage mechanism.

Key words: Folium Artemisiae Argyi, water extracted and volatile oil components, hepato-toxicity, oxidative damage mechanism

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