中国药物警戒 ›› 2011, Vol. 8 ›› Issue (6): 321-322.

• 基础研究 •    下一篇

紫景天中熊果苷的含量测定

张海丰1, 孙健2, 滕坤1   

  1. 1. 通化师范学院,吉林 通化 134000;
    2. 中国中医科学院医学实验中心,北京 100700
  • 收稿日期:2015-08-27 修回日期:2015-08-27 出版日期:2011-06-10 发布日期:2015-08-27
  • 作者简介:张海丰,男,博士,中药化学与新药基础研究。E-mail:dragon1976999@163.com。
  • 基金资助:
    紫景天抗肿瘤活性成分研究,吉教科合字[2009]第攻14号

Determination of Arbutin in Sedum Purpureum by HPLC

ZHANG Hai-feng1, SUN Jian2, TENG Kun1   

  1. 1. Tonghua Normal University, Jilin Tonghua 134000, China;
    2. Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2015-08-27 Revised:2015-08-27 Online:2011-06-10 Published:2015-08-27

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摘要： 目的 建立HPLC测定紫景天中熊果苷含量的方法。方法 采用Agilent XDB-C18色谱柱（4.6mm×150mm,5 m）,以甲醇-水（20 80）为流动相,流速：0.8mL·min-1,柱温为室温,检测波长为282nm。结果 熊果苷进样量在5~160μg范围内线性关系良好,r=0.9998,平均加样回收率为101.67%,RSD为3.06%（n=6）。结论 该方法结果准确、灵敏快速、重现性好,可用于紫景天中熊果苷含量的测定。

关键词: 紫景天, 熊果苷, 高效液相色谱

Abstract: Objective To establish a HPLC method for the determination of arbutin in Sedum Purpureum. Methods Agilent XDB C18(4.6mm×150mm, 5μm) column was applied, using methanol-water(20 80) as mobile phase with the rate of 0.8mL·min-1. The column temperature was room temperature and the detective wavelength was 282 nm. Results A good linearity was obtained over the range of 5~160μg for arbutin(r=0.9998). The average recovery(n=6) was 101.67%(RSD=3.06%). Conclusion The method is practical, convenient, accurate, and can be used as the determination of arbutin in Sedum Purpureum.

Key words: Sedum purpureum, Arbutin, HPLC

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