中国药物警戒 ›› 2011, Vol. 8 ›› Issue (4): 199-201.

• 养血生发胶囊毒性实验研究 • 上一篇    下一篇

HPLC法测定养血生发胶囊中二苯乙烯苷、大黄素、大黄素甲醚的含量

郑丽娜1,2, 任海勇3, 张作平3, 孙蓉1   

  1. 1. 山东省中医药研究院,山东 济南 250014;
    2. 天津中医药大学,天津 300193;
    3. 济南杏林生物技术有限公司,山东 济南 250101
  • 收稿日期:2015-08-27 修回日期:2015-08-27 出版日期:2011-04-10 发布日期:2015-08-27
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,药药理与毒理研究。E-mail：sunrong107@163.com
  • 作者简介:郑丽娜,女,在读硕士研究生,药物分析与中药毒代研究。
  • 基金资助:
    国家重点基础研究发展计划（973）中医基础理论专项资助项目（2009CB522802）;山东省科技平台建设项目课题（2008GG2NS02021）

Determination of the Contents of 2,3,5,4' -tetrahydroxystilbene 2-O-β-D-Glucopyranoside,Emodi and Physcion in Yangxue Shengfa Capsules by HPLC

ZHENG Li-na1,2, REN Hai-yong3, ZHANG Zuo-ping3, SUN Rong1   

  1. 1. Shandong Academy of Chinese Medicine, Shandong Jinan 250014, China;
    2. Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China;
    3. Jinan Xinglin Biology Technology Limited Company, Shandong Jinan 250101, China
  • Received:2015-08-27 Revised:2015-08-27 Online:2011-04-10 Published:2015-08-27

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摘要： 目的 建立高效液相色谱法测定养血生发胶囊中二苯乙烯苷、大黄素、大黄素甲醚含量的检测方法,从指标成分角度来探讨养血生发胶囊毒性与何首乌的关系,并且为养血生发胶囊的质量研究提供依据。方法 采用高效液相色谱法,Hypersil C18柱（4.6mm×250mm,5μm）;测定二苯乙烯苷采用流动相：乙腈-水（19 81）,检测波长：320nm; 测定大黄素、大黄素甲醚采用流动相：甲醇-0.1%磷酸溶液（80:20）,检测波长：254nm。结果 二苯乙烯苷在0.005~0.5mg/mL,大黄素在0.00168~0.084mg/mL,大黄素甲醚在0.00104~0.052 mg/mL,范围内呈良好线性关系;平均回收率：二苯乙烯苷98.66%,大黄素为95.80%,大黄素甲醚为97.70%;RSD：二苯乙烯苷为1.23%,大黄素为1.63%,大黄素甲醚为1.89%。结论 本方法检测快速,定量准确,线性关系、重现性、稳定性较好、回收率较高,可用于养血生发胶囊的含量测定。初步证实养血生发胶囊的毒性可能与何首乌有关,蒽醌类物质可能是养血生发胶囊的毒性成分。

关键词: 养血生发胶囊, 二苯乙烯苷, 大黄素, 大黄素甲醚, 高效液相色谱法

Abstract: Objective To develop an HPLC method for determination of 2,3,5,4'-tetrahydroxystilbene 2-O-β-D-Glucopyranoside, emodin and physcion in Yangxue Shengfa Capsules, to explore the relationship of toxicity of Yangxue Shengfa Capsules and Radix Polygoni multiflori from the perspective of index constituents, and to provide evidence for the quality reseach of Yangxue Shengfa Capsules. Methodse HPLC, Hypersil C18 column(4.6mm× 250mm, 5μm)was adopted; The solvent system consisting of acetonitrile-water(19:81) was used in the determination of 2,3,5,4'-tetrahydroxystilbene 2-O-β-D-Glucopyranoside,and the detection wavelength was at 320nm. The solvent system consisting of methanol-0.1% phosphoric acid(80:20)was used in the determination of emodin and physcion, and the detection wavelength was at 254nm. Results The linear ranges of 2,3,5,4'-tetrahydroxystilbene 2-O-β-D-Glucopyranoside, emodin and physcion were 0.005~0.5mg/mL, 0.00168~0.084mg/mL, and 0.00104~0.052 mg/mL,respec-Tively. The average recoveries were 98.67% (RSD=1.23%), 95.80% (RSD=1.63%), 97.70%(RSD=1.89%), respectively. Conclusion The Methodse are rapid, accurate, and with good linear relationship, good reproducibility, good stability, and high recovery, can be used for the determination of Yangxue Shengfa Capsules. It is initially confirmed that the toxicity of Yangxue Shengfa Capsules may be related with Radix Polygoni multiflori, and anthraquinone may be toxic components.

Key words: Yangxue Shengfa Capsules, 2,3,5,4'-tetrahydroxystilbene 2-O-β-D-Glucopyranoside, emodin, physcion, high performance liquid chromatography

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