中国药物警戒 ›› 2011, Vol. 8 ›› Issue (3): 154-157.

• 中药提纯技术研究 • 上一篇    下一篇

超声波优化栀子苷提取的工艺研究

欧阳薇, 乐龙, 王志祥， 张轻轻, 党蓓蕾   

  1. 中国药科大学制药工程教研室,江苏 南京 210009
  • 收稿日期:2015-08-27 修回日期:2015-08-27 出版日期:2011-03-10 发布日期:2015-08-27
  • 通讯作者: 王志祥,男,教授,博士生导师,制药工程教学和研究。E-mail:chinawzx@sohu.com
  • 作者简介:欧阳薇,女,在读硕士研究生,制药工程。

Optimization of Ultrasonic Extraction Conditions of Geniposide

OUYANG Wei, YUE Long, WANG Zhi-xiang, ZHANG Qing-qing, DANG Bei-lei   

  1. Dept. of Pharmaceutical Engineering, China Pharmaceutical University, Jiangsu Nanjing 210009
  • Received:2015-08-27 Revised:2015-08-27 Online:2011-03-10 Published:2015-08-27

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摘要： 目的 研究栀子苷的最佳超声提取条件。方法 采用响应面分析法优化栀子苷的超声提取条件,在单因素实验基础上利用BBD组合设计Box-Behnken 中心组合设计拟合试验条件。结果 以栀子苷提取率为响应值进行多元二次响应回归分析,可得超声提取栀子苷的最佳工艺条件为：超声功率 750.87 W、提取时间 46.57 min、溶剂浓度 52.06 %、溶剂体积（料液比）1:64.09 g·mL-1。此最优条件下栀子苷提取率预测为 36.16 mg·g-1,验证值为 35.49 mg·g-1,与预测值的相对误差为 1.85%。结论 此最优工艺条件具有一定的实际应用价值,对工业化生产有一定的指导意义。

关键词: 栀子苷, 超声提取, 响应曲面分析法

Abstract: Objective To study the technology of extracting geniposide from Gardenia jasminoides Ellis by ultrasound extraction. Methods Based on the single-factor experiments, a four-factor-three-level experiment design was developed by Box-Behnken central composite design method. Multi-quadratic equation was established with the yield of geniposide as response values. Results The optimum extraction condition was obtained as follows: ultrasound power 750.87 W, extraction time 46.57 min, ethanol concentration 52.06 % and ethanol volume(ratio of solvent to solid) 1:64.09 g·mL-1. The predictive maximum extraction yield was 36.16 mg·g-1 while the average experimental value was 35.49 mg·g-1, with the relative error of 1.85%. Conclusion The method is reliable, and can be used for industrial production.

Key words: geniposide, ultrasound extraction, response surface methodology

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