中国药物警戒 ›› 2010, Vol. 7 ›› Issue (8): 462-464.

• 柴胡毒性影响因素研究 • 上一篇    下一篇

南柴胡不同炮制品皂苷类物质含量及急性毒性实验比较研究

黄伟1,2, 孙蓉1*, 张作平3, 任海勇3   

  1. 1山东省中医药研究院,山东 济南 250014;
    2山东中医药大学,山东 济南 250355;
    3济南杏林生物技术有限公司,山东 济南 250101
  • 收稿日期:2016-02-22 修回日期:2016-02-22 出版日期:2010-08-08 发布日期:2016-02-22
  • 通讯作者: 孙蓉,女,研究员,硕士生导师,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:黄伟, 男,在读硕士研究生,中药药理与毒理。
  • 基金资助:
    国家自然科学基金项目（30672649）; 山东省科技平台建设项目（2008GG2NS02021）; 山东省科技公关关键技术研究课题（2007GG2NS02073）; 山东省国际合作引智项目（L20083700336）; 国家863科技攻关计划课题（2004BA721A15）; 国家重点基础研究发展计划 （973）中医基础理论专项资助项目 （2009CB522802）

Comparative Study on the Content of Saikosaponin Substances and Acute Toxicity of Radix Bupleuri Scorzoneraefolii and its Processed Products

HUANG Wei1,2, SUN Rong1,*, ZHANG Zuo-ping3, REN Hai-yong3   

  1. 1Shandong Research Academy of TCM,Shandong Jinan 250014, China;
    2Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355,China;
    3Jinan Xinglin Biology Techonology Corporation, Shandong Jinan 250101, China
  • Received:2016-02-22 Revised:2016-02-22 Online:2010-08-08 Published:2016-02-22

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摘要： 目的 对南柴胡不同炮制品中皂苷类物质的含量和急性毒性大小进行比较研究,探讨炮制对南柴胡皂苷类物质含量与毒性的影响。方法 运用HPLC法、经典急性毒性实验法,进行南柴胡不同炮制品中柴胡皂苷a的含量测定及急性毒性比较研究。结果 南柴胡生品、清炒品、醋炙品、酒炙品和蜜炙品不同炮制品中柴胡皂苷a的百分含量依次为0.46%、0.16%、0.22%、0.22%、0.32%。南柴胡生品,清炒品,醋炙品,酒炙品和蜜炙品没有出现死亡,测得最大给药量（MLD）值按含生药量计算分别为25.2、28.8、26.4、26.0、32.4 g·kg-1,分别相当于临床70 kg人每公斤体重日用量的196、224、205、233、252倍,连续观察14天,小鼠一般状况良好,无一死亡。 结论 南柴胡不同炮制品中柴胡皂苷a含量和毒性各不相同,且各样品均未出现死亡,其最大给药量显示南柴胡的各炮制品基本安全低毒,提示炮制对南柴胡药材柴胡皂苷类物质含量和安全剂量范围大小有一定影响。

关键词: 南柴胡, 炮制品, 柴胡皂苷, 小鼠, 急性毒性

Abstract: Objective To compare the content of saikosaponin and the acute toxicity on Radix Bupleuri Scorzoner-aefolliand its processed products, and discuss the effect of processing on the content and toxicity of Bupleurum saikosaponin substances. Methods HPLC method and the classical method of acute toxicity were used to determine the content of saikosaponin a and the acute toxicity of the Radix Bupleuri Scorzoneraefolliand its processed products, and compare the content and the acute toxicity. Results The content of saikosaponin a on the different processed products which included health products, fried products, vinegar products, wine products and honey roasted products was separately 0.46%,0.16%,0.22%,0.22%,0.32%. The health products, fried products, vinegar products, wine products and honey roasted products of Radix Bupleuri Scorzoneraefolli did not appear dead. Calculated by crude drug content, the MLD were respectively 25.2,28.8,26.4,26.0,32.4g·kg-1,which were respectively equal to 196,224,205,233,252 times of 70kg people's daily dried medicinal herb expenses. Making continuous observation of 14 days, mice were generally in good condition,and no one died. Conclusion The content of saikosaponin a and the acute toxicity of the Radix Bupleuri Scorzoneraefolliand its processed products were different, and no death occurred in each sample,the MLD showed the basic security and low toxicity of the Radix Bupleuri Scorzoneraefolliand its processed products, suggesting that processing has some influence on the content of the saikosaponin substances and the safe dose range of Radix Bupleuri Scorzoneraefolli herbs.

Key words: Radix Bupleuri Scorzoneraefolli, processed products, Bupleurum saikosaponin, mice, acute toxicity

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