中国药物警戒 ›› 2010, Vol. 7 ›› Issue (8): 465-468.

• 柴胡毒性影响因素研究 • 上一篇    下一篇

毒性导向下的柴胡总皂苷精制工艺研究

黄伟1,2, 孙蓉1*, 张作平3, 任海勇3, 吕莉莉1   

  1. 1山东省中医药研究院,山东 济南 250014;
    2山东中医药大学,山东 济南 250355;
    3济南杏林生物技术有限公司,山东 济南 250101
  • 收稿日期:2016-02-22 修回日期:2016-02-22 出版日期:2010-08-08 发布日期:2016-02-22
  • 通讯作者: 孙蓉,女,硕士生导师,研究员,中药药理与毒理。E-mail：sunrong107@163.com
  • 作者简介:黄伟,男,在读硕士研究生,中药药理与毒理。
  • 基金资助:
    国家自然科学基金项目（30672649）; 山东省科技平台建设项目（2008GG2NS02021）; 山东省科技公关关键技术研究课题（2007GG2NS02073); 山东省国际合作引智项目（L20083700336）; 国家863科技攻关计划课题（2006GG2202065）; 国家重点基础研究发展计划 （973）中医基础理论专项资助项目 （2009CB522802）; 国家重点基础研究发展计划 （973）中医基础理论专项资助项目 （2009CB522802）

Study on the Refining Process of the Total Bupleurum Saikosaponin under the Guidance of Toxicity

HUANG Wei1,2, SUN Rong1,*, ZHANG Zuo-ping3, REN HAI-yong3, LV Li-li1   

  1. 1Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355,China;
    3Jinan Xinglin Biology Techonology Corporation, Shandong Jinan 250101, China
  • Received:2016-02-22 Revised:2016-02-22 Online:2010-08-08 Published:2016-02-22

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摘要： 目的 通过小鼠急性毒性比较研究,探讨不同精制工艺对毒性物质基础柴胡总皂苷的富集程度。方法 参照经典的急性毒性实验方法,进行柴胡总皂苷粗提取物、柴胡总皂苷水洗脱液和柴胡总皂苷醇洗脱液对小鼠的急性毒性比较研究。结果 按总皂苷含量计算,测得柴胡总皂苷粗提取物LD15为0.3 g·kg-1,MTD为 0.48 g·kg-1;测得柴胡总皂苷水洗脱液LD10为0.83 g·kg-1,MTD为 1.324 g·kg-1;测得柴胡总皂苷醇洗脱液LD100为12.4 g·kg-1。结论 上述不同精制工艺样品的柴胡总皂苷富集程度为：柴胡总皂苷醇洗脱液 > 柴胡总皂苷水洗脱液 > 柴胡总皂苷粗提物 ;上述不同精制工艺样品的小鼠急性毒性大小为：柴胡总皂苷醇洗脱液 > 柴胡总皂苷粗提物 > 柴胡总皂苷水洗脱液,故确定醇洗脱工艺为富集柴胡总皂苷的最佳精制工艺。

关键词: 柴胡总皂苷粗提取物, 醇洗脱液, 水洗脱液, 小鼠, 急性毒性

Abstract: Objective By comparing the acute toxicity of mice, to explore the enrichment of different purification process on the total Bupleurum saikosaponin which is the mainly toxical substance basis of Bupleurum. Methods Make the comparing study on mice's acute toxicity of the crude extracts of total Bupleurum saikosaponin, the water elution of total Bupleurum saikosaponin and the alcohol elution of toatal Bupleurum saikosaponin in accordance with classical acute toxicitytest Methods. Results Caculated by total saikosaponin, the LD15 of the crude of total Bu-pleurum saikosaponin is 0.3g·kg-1,and the MTD is 0.48 g·kg-1; the LD10 of the water elution of total Bupleurum saikosaponin is 0.83g·kg-1,and the MTD is 1.324g·kg-1; the LD100 of the alcohol elution of total Bupleurum saikosaponin is 12.4g·kg-1. Conclusion The enrichment of different purification process on the total Bupleurum saikosaponin: the alcohol elution of toatal Bupleurum saikosaponin > the water elution of total Bupleurum saikosaponin > the crude extracts of total Bupleurum saikosaponin;the acute toxicity of different purification process on the total Bupleurum saikosaponin: the alcohol elution of toatal Bupleurum saikosaponin > the crude extracts of total Bupleurum saikosaponin> the water elution of total Bupleurum saikosaponin, so we determine that the best refining process of enriching the total Bupleurum saikosaponin is alcohol elution process.

Key words: the crude extracts of total Bupleurum saikosaponin, the alcohol elution, the water elution, mice, acute toxicity

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