中国药物警戒 ›› 2010, Vol. 7 ›› Issue (10): 579-584.

• 基础及临床研究 • 上一篇    下一篇

香加皮不同组分对小鼠急性毒性实验比较研究

孙蓉1,鲍志烨2,黄伟2,吕莉莉1,张作平3,任海勇3   

  1. 1.山东省中医药研究院,山东济南250014；
    2.山东中医药大学,山东济南250355；
    3.济南杏林生物技术有限公司,山东济南250101
  • 收稿日期:2010-04-09 修回日期:2016-03-12 出版日期:2010-10-08 发布日期:2016-03-09
  • 作者简介:孙蓉,女,研究员,硕士生导师,中药药理与毒理研究。

Experimental Comparison Study on Mice's Acute Toxicity of Different Composition in Cortex Periplocae

SUN Rong1, BAO Zhi-ye2, HUANG Wei2 ,LV Li-li1, ZHANG Zuo-ping3 ,REN Hai-yong3   

  1. 1.Shandong Research Academy of TCM, Shandong Jinan 250014, China;
    2.Shandong University of Traditional Chinese Medicine, Shandong Jinan 250355, China;
    3.Jinan Xinglin Biology Techonology Corporation, Shandong Jinan 250101, China
  • Received:2010-04-09 Revised:2016-03-12 Online:2010-10-08 Published:2016-03-09

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摘要： 目的 比较进行香加皮全组分、水提组分、醇提组分的急性毒性观察与评价,为临床用药的安全性提供依据。方法 别制备香加皮上述不同组分,按照经典小鼠急毒试验方法,用bliss 法计算半数致死量（LD50）,公斤体重法计算最大耐受量（MTD）,考察香加皮全组分（MTD）值、水提组分（LD50）值、醇提组分（LD50）值,连续给药观察14 天,记录各组动物体重变化,毒性症状谱及死亡情况。结果 香加皮药材全组分MTD 可达16.0 g·kg-1·d-1,相当于临床人用量的186.6 倍；香加皮水提组分LD50 值为93.578 g·kg-1·d-1,95%的可信限为89.149～98.484 g·kg-1·d-1,相当于临床人用量的1 091.8 倍,香加皮醇提组分LD50值为61.388 g·kg-1·d-1,95%的可信限为57.466～65.521 g·kg-1·d-1,相当于临床人用量的716.2 倍。通过小鼠急毒实验观察：肢体麻痹、腹泻、抽搐、呼吸抑制是香加皮不同组分主要的急性毒性症状,香加皮水提、醇提组分小鼠死亡多发生于48 小时之内。结论 香加皮不同组分毒性大小顺序依次为醇提组分、水提组分、全组分。

关键词: 香加皮, 全组分, 水提组分, 醇提组分, 急性毒性

Abstract: Objective To compare the acute toxicity of different components in Cortex Periplocae on mice which is all components、water extraction components and alcohol extraction components, make an observation and evaluation of acute toxicity to Cortex Periplocae herbs in order to provide a basis for safe and reasonable medication in clinical. Methods Respectively prepare the all components、water extraction components and alcohol extraction components, and compare the acute toxicity of different components in Cortex Periplocae on mice in accordance with classical acute toxicity test methods, and the experimental datas are calculated LD50 with the Bliss method and MTD method, with the continuous administration and observation for 14 days. Results the all components in Cortex Periplocae is unable to make LD50, MTD Results calculated in accordance with crude drug content is respectively 16.0g·kg-1·d-1,which is equal to 186.6 times of 70kg people's daily dried medicinal herb expenses. The LD50 of water extraction components and alcohol extraction components in Cortex Periplocae are 93.578g·kg-1·d-1 and the 95% confidence limit is 89.149～98.484g·kg-1·d-1 which is equal to1 091.8 times of 70kg people's daily dried medicinal herb expenses and 61.388g·kg-1·d-1 and the 95% confidence limit is 57.466～65.521g·kg-1·d-1 which is equal to 716.2 times of 70kg people's daily dried medicinal herb expenses. The main toxical symptoms and signs are the convulsions, tetraplegia, diarrhoea and the respiratory depression, the deaths mainly occurred in less than 48 hours used by the all components、water extraction components and alcohol extraction components. Conclusion The acute toxicity of different components in Cortex Periplocae on mice is: alcohol extract>water extract>all-components.

Key words: Cortex Periplocae , all-components, water extract, alcohol extract, acute toxicity

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