中国药物警戒 ›› 2025, Vol. 22 ›› Issue (10): 1094-1099.
DOI: 10.19803/j.1672-8629.20250370

• 何首乌质量研究专栏 • 上一篇    下一篇

基于Q-Exactive-Orbitrap MS的何首乌叶成分解析及蒸制原理研究

王燕1, 张凯1, 牛闯1, 刘伟1, 金海娜1, 汪祺2,*   

  1. 1北京卫生职业学院中药与康复系,北京 101101;
    2中国食品药品检定研究院中药民族药检定所,北京 100050
  • 收稿日期:2025-06-16 出版日期:2025-10-15 发布日期:2025-10-20
  • 通讯作者: *汪祺,女,博士,研究员,中药质量与安全性评价。E-mail: sansan8251@sohu.com
  • 作者简介:王燕,女,硕士,副教授,中药鉴定与质量评价。
  • 基金资助:
    国家自然科学基金资助项目（82374033）; 北京卫生职业学院筑基计划科研课题《中药何首乌质量调研分析》（2024009）

Components of Polygonum multiflorum Thunb. Leaves and Methods of Steaming Based on High-Resolution Q-Exactive-Orbitrap MS

WANG Yan1, ZHANG Kai1, NIU Chuang1, LIU Wei1, JIN Haina1, WANG Qi2,*   

  1. 1Beijing Health Vocational College, Department of Traditional Chinese Medicine and Rehabilitation, Beijing 101101, China;
    2National Institutes for Food and Drug Control, Institute for Control of Chinese Traditional Medicine and Ethnic Medicine, Beijing 100050, China
  • Received:2025-06-16 Online:2025-10-15 Published:2025-10-20

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摘要： 目的 解析何首乌叶的化学成分,并比较蒸制前后化学成分变化,初步探索蒸制对其化学成分的影响。方法 采用Waters Acquity UPLC BEH-C18色谱柱（100 mm×2.1 mm,1.7 μm）,以0.1%甲酸水溶液（A）-乙腈（B）为流动相进行梯度洗脱,用四极杆-静电场轨道阱组合式高分辨质谱仪（Q-Exactive-Orbitrap MS）采集数据分析,利用标品对其化学成分进行定性和相对定量分析,同时与文献主要化学成分比对结果。结果 何首乌叶及其蒸制品鉴定成分30种,蒸制前后特征性成分相对含量变化差异较大,蒸制后二苯乙烯苷、蒽醌类成分含量降低,鞣质类含量增加,其中特征性成分二苯乙烯苷类含量降低50%以上。结论 何首乌叶含有二苯乙烯苷、蒽醌、黄酮等成分,与块根中特征性成分一致,蒸制后各成分含量有变化,为其药用价值研发及内服的安全性提供参考。

关键词: 何首乌叶, 二苯乙烯苷, 蒽醌, 黄酮, 蒸制, 四极杆-静电场轨道阱组合式高分辨质谱仪

Abstract: Objective To analyze the chemical constituents of Polygonum multiflorum Thunb. Leaves, observe the changes in chemical constituents steaming, and to explore the effect of steaming on the chemical constituents. Methods The Waters Acquity UPLC BEH-C18 column (100 mm×2.1 mm, 1.7 μm) was used, and the mobile phase was composed of 0.1% formic acid water solution (A) - acetonitrile (B) under gradient elution. Q-Exactive-Orbitrap MS was used for data acquisition and analysis. The chemical constituents were qualitatively and relatively quantitatively analyzed by using standard products, and the results were compared with what was reported in literature. Results Thirty components were identified in Polygonum multiflorum Thunb. leaves and their steamed products. The relative contents of characteristic components changed significantly after steaming. The contents of stilbene glycosides and anthraquinones decreased, especially the former, whose content plummeted by more than 50%, while that of tannins increased. Conclusion Polygonum multiflorum Thunb. leaves contain such components as stilbene glycosides, anthraquinones and flavonoids, which are consistent with the characteristic components in the roots. The contents of components change after steaming. This study is expected to provide data for the tapping of its medicinal value and ensuring the safety of the drug for internal use.

Key words: Polygonum multiflorum Thunb. Leaves, Stilbene Glucoside, Anthraquinone, Flavone, Steaming Processing, Q-Exactive-Orbitrap MS

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