中国药物警戒 ›› 2025, Vol. 22 ›› Issue (11): 1236-1240.
DOI: 10.19803/j.1672-8629.20241031

• 基础与临床研究 • 上一篇    下一篇

基于SEM技术与UPLC色谱法研究东莨菪碱在洋金花中的分布

汪济舟1,2, 徐璐1,2, 朱利利1, 薛淑娟1,2,3, 陈随清1,2,3,4*, 孙孝亚1,2,3#   

  1. 1河南中医药大学药学院,河南 郑州 450046;
    2河南省中药资源与中药化学重点实验室,河南 郑州 450046;
    3豫药全产业链研发河南省协同创新中心,河南 郑州 450046;
    4呼吸疾病中医药防治省部共建协同创新中心,河南 郑州 450046
  • 收稿日期:2024-12-24 出版日期:2025-11-15 发布日期:2025-11-14
  • 通讯作者: *陈随清,男,教授,博导,中药品种整理与质量标准研究。E-mail：suiqingchen0371@163.com,#为共同通信作者。
  • 作者简介:汪济舟,男,硕士,中药鉴定资源与评价。
  • 基金资助:
    国家重点研发计划（2023YFC3504005）; 国家科技基础资源调查专项（2018FY100802-03）; 财科〔2024〕8号河南省创新生态支持专项

Distribution of Scopolamine in Daturae Flos Based on SEM Technology and UPLC Chromatography

WANG Jizhou1,2, XU Lu1,2, ZHU Lili1, XUE Shujuan1,2,3, CHEN Suiqing1,2,3,4*, SUN Xiaoya1,2,3#   

  1. 1School of Pharmacy, Henan University of Chinese Medicine, Zhengzhou Henan 450046, China;
    2Henan Provincial Key Laboratory of Chinese Medicine Resources and Chinese Medicine Chemistry, Zhengzhou Henan 450046, China;
    3Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao, Zhengzhou Henan 450046, China;
    4Collaborative Innovation Center for Chinese Medicine and Respiratory Diseases Co-Constructed by Henan Province & Education Ministry of China, Zhengzhou Henan 450046, China
  • Received:2024-12-24 Online:2025-11-15 Published:2025-11-14

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摘要： 目的 采用定量分析方法,结合超显微结构分析,探究东莨菪碱在洋金花中的分布。方法 将洋金花分为全花、下段、中段、上段、花萼、子房和花冠七类样品,使用扫描电子显微镜（SEM）对七类样品进行超显微结构的观察分析,并基于UPLC色谱法对其主要活性成分东莨菪碱的含量进行测定,得到七类样品中东莨菪碱的相对含量,并对比分析,判断洋金花中东莨菪碱的大致分布情况。结果 通过SEM观察,发现洋金花的分泌细胞如腺毛、气孔,和晶体主要集中在子房及其附近的部分,七类样品的东莨菪碱相对含量高低顺序如下：子房>下段>全花>上段>花冠>中段>花萼。因为洋金花各个部位中东莨菪碱的含量均存在差异,体积占比最大的花冠、花萼中含量远低于子房,而洋金花药材具有体积较大、质脆易碎等特点,因此在运输与储存中很难保持其完整性,且洋金花为有毒药材,这些特点对洋金花的临床药效及用药安全产生一定影响。结论 本研究揭示了现行洋金花质量评价体系存在一定的优化空间,其研究结果可为提升该药材的临床疗效稳定性与用药安全性提供参考。

关键词: 洋金花, 东莨菪碱, 超高效液相色谱, 超显微结构, 电子显微镜, 活性成分分布

Abstract: Objective To study the distribution of scopolamine in Daturae Flos via quantitative analysis combined with ultra-microscopic structural analysis. Methods The plant was divided into seven sections: the whole flower, lower segment, middle segment, upper segment, calyx, ovary and corolla. Scanning electron microscopy (SEM) was employed to observe and analyze the ultra-microscopic structures of the seven parts. Results SEM observation found that the secretory cells of Daturae Flos, such as glandular hairs, stomata and crystals, were concentrated in the ovary and its adjacent parts. The content of scopolamine was the highest in the ovary, followed by the lower segment, whole flower, upper segment, corolla, middle segment and calyx. The content of scopolamine varied across these parts. The corolla and calyx, which accounted for a large proportion of the volume, had lower contents than in the ovary. The large size and fragility of the decoction pieces of Daturae Flos made it difficult to maintain their integrity during transportation and storage. In addition, Daturae Flos was a toxic medicinal material. These properties could impact the clinical efficacy and safety of Daturae Flos. Conclusion This study reveals where the current quality evaluation system for Daturae Flos can be improved, and the research results are of referential value for ensuring the clinical efficacy and safety of medication of this medicinal material.

Key words: DATURAE FLOS, Scopolamine, UPLC, Ultra-Microscopic Structure, Electron Microscope, Distribution of Active Ingredients

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