中国药物警戒 ›› 2024, Vol. 21 ›› Issue (3): 253-256.
DOI: 10.19803/j.1672-8629.20230797

• 基于多模型、多靶点的中药抗感染药效及机制研究专栏（二） • 上一篇    下一篇

BD-77雾化吸入给药对肺炎支原体感染小鼠的治疗作用

孙静1, 赵荣华1,Δ, 郭姗姗1, 高双荣1, 包蕾1, 耿子涵1, 李舒冉1, 孙绮悦1, 徐洲2, 覃广源3, 潘玉杰3, 谭秋霞3, 龙忠义3, 黄成钢2,#, 崔晓兰1,*   

  1. 1中国中医科学院中药研究所,北京 100700;
    2中国科学院上海药物研究所,上海 201203;
    3贵州百灵企业集团制药股份有限公司,贵州 安顺 561099
  • 收稿日期:2023-12-21 出版日期:2024-03-15 发布日期:2024-03-18
  • 通讯作者: *崔晓兰,女,博士,研究员,中药抗病毒及抗感染药理。E-mail: xlcui@icmm.ac.cn;#为共同通信作者。
  • 作者简介:孙静,女,博士,副研究员,中药抗病毒及抗感染药理。Δ为并列第一作者。
  • 基金资助:
    国家自然科学基金资助项目（82141206）

Therapeutic effect of aerosol inhalation of BD-77 against mycoplasma pneumoniae infection in mice

SUN Jing1, ZHAO Ronghua1,Δ, GUO Shanshan1, GAO Shuangrong1, BAO Lei1, GENG Zihan1, LI Shuran1, SUN Qiyue1, XU Zhou2, QIN Guangyuan3, PAN Yujie3, TAN Qiuxia3, LONG Zhongyi3, HUANG Chenggang2,#, CUI Xiaolan1,*   

  1. 1Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    2Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;
    3Guizhou Bailing Enterprise Group Pharmaceutical Co., LTD., Anshun Guizhou 561099, China
  • Received:2023-12-21 Online:2024-03-15 Published:2024-03-18

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摘要： 目的 评价BD-77雾化吸入给药对肺炎支原体肺炎小鼠模型的治疗作用。方法 Balb/c小鼠按照体质量随机分为正常对照组、模型对照组、阿奇霉素对照组（42 mg·kg-1·d-1）、BD-77高剂量组（75 mg·mL-1,雾化15 min）、BD-77低剂量组（37.5 mg·mL-1,雾化15 min）。以肺炎支原体滴鼻感染建立小鼠支原体肺炎模型,雾化给药4 d后,通过小鼠体重及肺重计算肺指数和肺指数抑制率,酶联免疫吸附试验（ELISA）检测肺组织中白介素-6（IL-6）、白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）含量及血清中C反应蛋白（CRP）水平,苏木精-伊红（HE）染色评估小鼠肺组织病理学变化,全面评价BD-77对小鼠支原体肺炎的治疗作用。结果 BD-77 2个剂量组雾化吸入给药15 min均可显著降低小鼠肺指数,减轻肺部炎症病变,降低肺组织中IL-6、IL-1β、TNF-α含量及血清CRP水平。结论 BD-77雾化吸入可治疗支原体感染小鼠肺炎,本研究为BD-77开发作为防治肺炎支原体肺炎的药物提供了实验室数据支持。

关键词: BD-77, 肺炎支原体, 雾化吸入, 炎症因子, 肺指数, 肺指数抑制率, 小鼠

Abstract: Objective To evaluate the therapeutic effect of aerosol inhalation of BD-77 against mycoplasma pneumoniae (MP) infection in mice. Methods Balb/c mice were randomly divided into the normal control group, model control group, azithromycin control group (42 mg·kg-1·d-1), BD-77 high dose group (75 mg·mL-1, 15 min), and BD-77 low dose group (37.5 mg·mL-1, 15 min). The mouse model of mycoplasma pneumoniae pneumonia (MPP) was induced by intranasal infection with MP. After four consecutive days of aerosol inhalation, the therapeutic effect of BD-77 was evaluated based on the lung index, lung inhibition rate, and pathological changes of lung tissues. The contents of interleukin (IL)-6, IL-1β, tumor necrosis factor α (TNF-α) in lung tissues and the level of C reactive protein (CRP) in serum were also detected using ELISA method. Results Both doses of BD-77 aerosol administration could decrease the lung index and the release of IL-6, IL-1β and TNF-α in lung tissues, ameliorate pulmonary inflammation and reduce serum CRP levels. Conclusion Aerosol inhalation of BD-77 preparation is effective for MPP in mice. This study provides data for the development of BD-77 as a drug for prevention and treatment of MPP.

Key words: BD-77, mycoplasma pneumoniae, aerosol inhalation, inflammatory factor, lung index, lung inhibition rate, mouse

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