Abstract: ObjectiveTo build up RP-HPLC method for measuring homobarringtonie and related substances of its preparations, and to analyze the content of homobarringtonie related substances after different treatment. MethodsApply Germsil-P C18 chromatographic column (250×4.6 mm, 5 μm)and use 10 mmol·L-1 monopotassium phosphate as flow phase A while methanol as B. Apply gradient elution with the flow rate 1 mL·min-1, check the wavelength 288 nm with column temperature at 30 ℃. ResultsHomobarringtonie presented great linear relationship with peak area in the range of 2 ~30μg, with r=0.9996; when loading 20 μg homobarringtonie in 6 repeats, impurity A, impurity B, and homobarringtonie RSD were 3.28%, 4.69%, and 1.12%, respectively. ConclusionApplying this method can precisely measure the content of homobarringtonie, and related substances, such as impurity A and impurity B.

Key words: homobarringtonie, related substance, RP-HPLC