Effects of Astragalus on Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 in Rats of Acute Lung Injury

Abstract

Abstract: Objective To investigate the protective effects of Astragalus on acute lung injury and to explore the role of intercellular adhesion molecule^-1 (ICAM^-1) and vascular cell adhesion molecule^-1 (VCAM^-1) on acute lung injury in rats. Methods 30 Wistar rats were randomly divided into control group, model group and Astragalus group (n=10 , respectively). All rats were sacrificed 6 h after administration of Astragalus, removed lung tissue for pathological observation, and examined the expression of myeloperoxidase (MPO), superoxide dismutase (SOD) activity and ICAM^-1, VCAM^-1 in each group. Results Compared with control group, MPO activity and ICAM^-1, VCAM^-1 expression were significantly increased in model group and Astragalus group (P <0.05 or P <0.01 respectively).However, in Astragalus group, MPO activity and ICAM^-1, VCAM^-1 expression were significantly lower than that of model group (P <0.05 or P <0.01 respectively). Lung tissue SOD activity was significantly lower after lung injury(P <0.05 or P <0.01 respectively), but it was significantly enhanced after treatment with Astragalus (P <0.05). Conclusion Astragalus can play a protective effect on ALI, and the mechanism may relate to regulate the expression of ICAM^-1, VCAM^-1 and increase the activity of SOD.

Key words: Astragalus, acute lung injury, myeloperoxidase, superoxide dismutase, adhesion molecule

CLC Number:

R965

ZHOU Yi,LI Huo-ping,XIA Guang-ming,ZHANG Hui,JIN Zhao-xia,ZHOU Fan,SHI Su-ping. Effects of Astragalus on Intercellular Adhesion Molecule-1 and Vascular Cell Adhesion Molecule-1 in Rats of Acute Lung Injury[J]. Chinese Journal of Pharmacovigilance, 2015, 12(4): 197-199.

References

[1] 左军,刘文钊,胡晓阳,等.黄芪现代药理及临床研究进展[J].中医药信息,2014,31(1):111-112.
[2] 刘德丽,包华音,刘杨.近5年黄芪化学成分及药理作用研究进展[J].食品与药品，2014,16(1):67-70.
[3] 陶伍元.黄芪注射液对急性肺损伤大鼠HSP70、NF-KB表达的影响[D].广东：广东医学院硕士学位论文,2010:1-41.
[4] Su X, Bai C , Hong Q, et al.Effect of continuous hemofi l tration on he modyna mics , lung inflammation and pulmonary ede ma in a canine model of acute lung injury[J]. Intensive Care Med, 2003, 29(11): 2034 -2042.
[5] 陆月明.油酸致急性肺损伤动物模型析评[J].中华急诊医学杂志,2005,14 (1):81-83.
[6] 谢春燕,王顺和,陈利江. EAE大鼠脑中ICAM-1、VCAM-1的检测及意义[J].重庆医科大学学报,2005,30(2):224-227.
[7] Artigas A,Bernard G R,Carlet,et al.The American-European consensus conference on ARDS[J].Am J Respir Crit Care Med,1998,
157(4):1332-1347.
[8] 王和枚,丁日高,阮金秀.急性肺损伤的中性粒细胞作用机制研究进展[J].中国工业医学杂志，2002,15(6):348-352.
[9] 殷涛,王卫星,余佳,等. ICAM-l在大鼠急性胰腺炎肺损伤中的作用[J].中国药师,2009,12(5):551-553.
[10] Cybulski J,Kulakowski P,Budaj A,et al. Intravenous amio darone for cardioversion of recent-onset atrial fibrillation[J].Clin Cardiol,2003,26(7):329-335.
[11] Sun W,Watanabe Y,Wang Z Q.Expression and significance of ICAM-1 and its counter receptors LFA-1 and Mac-1 in experimental acute pancreatitis of rats[J].Word J Gastroenterol,2006,12(31):5005-5009.
[12] Grau G E,Mili N,Lou J N,et al.Phenotypic and functional analysis of Pulmonary microvascular endothetlial cells from Patients whit acute respiratory distress syndrome[J].Lab Invest,1996,74(4):761-770.
[13] 徐冰.粘附分子与UAP中医证候相关规律及黄芪多糖干预机理研究[D].北京：北京中医药大学博士论文，2012：1-149.
[14] 朱海燕.黄芪多糖对人心脏微血管内皮细胞再灌注损伤粘附分子表达及调控的研究[D].北京：北京中医药大学博士论文，2008：1-106.
[15] Millar A B,Foley N M,Singer M,et al.Tumor necrosis facter in bronchopulmonary secretions of patients with adult respiratory distress syndrome[J].Lancet,1989,2(8665): 712-714.
[16] Serra J A,Marschoff E R,Dominguez R O,et al.Comparison of the determination of superoxide dismutase and antioxidant capacity in neurological patients using two different procedures[J]. Clin Chim Acta,2000,301(1-2):87-102.