Abstract: Objective To develop a HPLC method for determination of martine, belamcandin and irisflorentin in Qiweiqingyan aerosol. Method The optimum HPLC condition was as follows: Agilent Zorbas Stablebond C18 column(4.6 mm×250 mm,5 μm) with gradient elution of acetonituile (A)- methanol(B)-0.1% phosphoric acid (C), detection wavelength:251 nm, column temperature: 30℃, flow rate:1mL?min-1. Results There were good linearity between peak areas and injection quantity of martine, belamcandin and irisflorentin within the range of 0.25～125 μg?mL-1, 0.10～101μg?mL-1, 0.12～234μg?mL-1.The average recoveries were between 98.5 %～100.6%. Conclusion The method could detect martine, belamcandin and irisflorentin in Qiweiqingyan aerosol. The HPLC measurement was reasonable, stable and accurate.

Key words: Qiweiqingyan aerosol, martine, belamcandin, irisflorentin;HPLC, determination of content