Identification of Hepatotoxic Components of Polygoni Multiflori Radix Based on Material Basis Combined with Heparg Cell Experiments

doi:10.19803/j.1672-8629.20240427

Abstract

Abstract: Objective To explore potential hepatotoxic components based on the differences in cytotoxicity between different batches of Polygoni Multiflori Radix(PM). Methods Ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-QQQ-MS/MS) was used for quantitative detection of PM while hierarchical cluster analysis was used to classify samples. The half inhibitory concentration (IC₅₀) of HepaRG cells was used as the index to evaluate the toxicity of categorized samples and to reveal the differences between different groups. Orthogonal partial least squares discriminant analysis was used to identify the differences in toxicity between different groups and screen for differential components. Results Twenty-two batches of samples were divided into categories A, B, and C based on common compounds. The IC₅₀ of HepaRG cells indicated that the three groups of samples were significantly different in terms of toxicity, which was the highest in group B, followed by group A and group C. The results of OPLS-DA suggested that the discrimination rate of the models between groups A, B, and C was good, and three potential toxic components with VIP>1 were obtained. Conclusion Different categories of PM samples exhibit differences in toxicity. trans-2, 3, 5, 4'-tetrahydroxystilbene-2-O-β-D-glucoside (THSG), emodin-8-O-β-D-glucoside (EG) and catechin are potential toxic components of PM.

Key words: Polygoni Multiflori Radix, Liver Injury, HepaRG Cells, HCA, OPLS-DA, THSG, EG, Catechin

CLC Number:

R917

LI Yanyi, WEN Hairuo, YANG Yanrong, MA Shuangcheng, WANG Qi. Identification of Hepatotoxic Components of Polygoni Multiflori Radix Based on Material Basis Combined with Heparg Cell Experiments[J]. Chinese Journal of Pharmacovigilance, 2024, 21(12): 1377-1381.

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https://zgywjj.magtechjournal.com/EN/Y2024/V21/I12/1377

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