Abstract: Objective To explore anti-tumor effect of five different extraction process from shenetang in vivo, so as to provide foundation to optimize the extraction process of the prescription. Methods The chemical composition and pharmacological activity of the various ingredients in the compound were summed up to determinate the extraction methods, the contents of material basis in different of extracts were detected.H22 tumor model in mice were built. Mice implanted withH22 were treated with five different extraction processes from shenetang for 7d, and then the tumor tissues, thymus glands and spleens were weighed. The tumor inhibitory rates, immune organ index were calculated to investigate the anti -tumor and immune regulatory effect of different extraction components from shenetang in mice implanted with tumor cells. Results The five different extraction components showed potent antitumor effect, it could raise the decreased immune organ index, in which group four shows better anti-tumor activity.Conclusion The effective components from shenetang have anti-tumor effect, group four with more anti-tumoractivity, which has good anti-tumor application prospects.

Key words: Shenetang, different extraction components, anti-tumor activity