Study on Fingerprint of Volatile Oil from Bupleurum Chinense by Capillary GC

Abstract

Abstract: Objective To establish the fingerprint of volatile oil from Bupleurum Chinense by capillary GC. Methods The fingerprint analysis of volatile oil from Bupleurum Chinense was made by capillary GC. The analysis was performed on an HP-5(30m×0.25mm×0.25μm). The column was maintained at 50℃ for 10 min then elevated to 240℃ at 20℃·min-1 and maintained for 40 min. Gasifieation temperature：250℃, carry gas:N2(99.99%), flow rate:2.0mL·min-1, inlet volumn: 0.5μL; spliting ratio：(40：1), FID, injector temperature：250℃. Results The GC fingerprints were established and the 16 common peaks were found and identified with GC in volatile oil of 6 batches of Bupleurum Chinense. The comparison on similarity degree of the crude drugs from various habitats has been carried on and the correlative coefficient was over 0.9. Conclusion The capillary GC method is so simple and reliable that it is capable of effectively controlling the quality of Bupleurum Chinense.

Key words: Bupleurum Chinense, volatile oil, capillary GC, fingerprint

CLC Number:

R282

SUN Ling, YANG Qian, WANG Jing-hu, HUANG Wei, SUN Rong. Study on Fingerprint of Volatile Oil from Bupleurum Chinense by Capillary GC[J]. Chinese Journal of Pharmacovigilance, 2011, 8(8): 462-464.

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