Effects of Bicyclol on the Inhibition of Gap Junctional Intercellular Communication by DDT in WB-F344 Cells

Abstract

Abstract: Abstract : Objective To determine the effects of bicyclol on dichloro diphenyl trichloroethane ( DDT ) -induced inhibition of gap junctional intercellular communication in WB -F344 rat liver epithelial cells and the mechanisms involved in the process. Methods The scrape loading/dye transfer(SL/DT) technique was used to assay the inhibition of GJIC induced by DDT and the preventive effects of bicyclol. The expression of total -Cx43, phosphor -Cx43, E -cadherin and β-catenin were assessed by Western blot. Immunofluorescence analysis was used to determine the subcellular distribution of Cx43, E -cadherin and β-Catenin. Results Two hours treatment of DDT could inhibit GJIC of WB-F344 cells in a dose-dependent manner. The cells were pre-incubated with bicyclol for 22h and this was followed by treatment with DDT for 2h, bicyclol could prevent the inhibition of GJIC induced by DDT. The reduction of high phosphor-Cx43 expression induced by DDT and the restoration of gap junction maybe contribute to the mechanisms of bicyclol. Both DDT and bicyclol showed no significant effect on expression, activity and cellular distribution of E -cadherin and β-catenin. Conclusion Bicyclol prevented the DDT -induced GJIC inhibition via regulating the expression of phosphor-Cx43 and restoring the gap junction. The inhibition of GJIC is a known cellular event associated with tumor promotion. Previous Results showed that bicyclol could prevent the hepatocarcinogenesis induced by diethylnitrosamine/phenobarbital. This paper suggested that bicyclol maybe also have the potential prevention to DDT induced tumorgenesis, which isa pesticide and strongly persists in the environment. Key words: bicyclol; WB-F344 cell; gap junctional intercellular communication; diohloro diphenyl trichloroethane(DDT)

Key words: bicyclol, WB-F344 cell, gap junctional intercellular communication, diohloro diphenyl trichloroethane(DDT)

CLC Number:

R975.5
R965

SUN Hua ,YU Ling-Hong ,WEI Huai-Ling ,ZHANG Dan . Effects of Bicyclol on the Inhibition of Gap Junctional Intercellular Communication by DDT in WB-F344 Cells [J]. Chinese Journal of Pharmacovigilance, 2013, 10(7): 385-389.

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