Abstract: Objective To study the protective effect of total glucoside (TGP) for infection on Concanavalin A(ConA)-induced acute liver injury in ICR mice. Methods ICR mice were given intravenously TGP injection 0.5～8mg·kg-1 for five days(one time/day). After the last administration 2h, the mice were injected intravenously ConA 20mg·kg-1 to establish the immunological liver damage model. All animals were killed on 16h after ConA treatment. The activities of ALT, AST and LDH were measured by automatic chemistry analyzer(TBA-40FR). Liver histopathological changes were examined by H.E. and light microscopy. Results ConA 20mg·kg-1 could induced significant liver damage, expressed in the higher serum ALT,AST,LDH activities and the apoptosis or inflammatory cell infiltration in liver tissue. TGP injection 0.5～4mg·kg-1 showed significantly protective effect to liver damage induced by ConA, which could reduce serum transaminase activities and prevent the pathological injury. The optimum dose is 1mg·kg-1. There wasa reduced efficacy when the dose is 8mg·kg-1. While at this dose, there are no obvious toxicity. Conclusion TGP injection showed a significant protective effect on ConA-induced liver injury in mice. Its effective dose is low and the therapeutic activity will cut down when the dose is higher than 8mg·kg-1. It should be careful to the dose choice in clinical trial.

Key words: total glucosides, injection, Con A, liver injury