Abstract: Objective To explore the feasible preparation process of β-cyclodextrin inclusion with volatile oil of Olibanum and Myrrha and the best conditions, and verify its analgesic effect. Methods To extract volatile oil of Olibanum and Myrrha, compare the rate of yield and utilization whereas the different proportion of inclusion, thus to optimize the preparation process of β-CD inclusion compound. Mice writhing experiment was taken to explore the analgesic effect of crude powder and β-CD inclusion Compound respectively. Results The optimization ratio of volatile oil and β-CD inclusion was 1:10, and the yield rate of inclusion compound was greater than 97%, and the utilization rate was more than 92%. The crude powder and β-CD inclusion Compound respectively had an inhibitory effect on mice writhing induced by acetic acid. Conclusion The process of β-CD inclusion with volatile oil of Olibanum and Myrrha was possible without affecting its analgesic effect.

Key words: Olibanum+Myrrha crude powder, β-cyclodextrin inclusion with volatile oil of Olibanum and Myrrha, analgesic effect