Abstract: Objective To reach the influences of different extracts from Cassiae Semen on "dose-toxicity" relationship and "time-toxicity" relationship of hepatotoxicity and nephrotoxicity in mice by serum biochemical indexes and pathological. Methods Mice were grouped according to different time or dose points, and given all-components, water extracted components and alcohol extracted components by gavage respectively. The ratio of liver and kidney to body was measured, the contents of ALT、AST、Cr、BUN and TNF-α related to hepatotoxicity and nephrotoxicity in serum were detected. Results The "time- toxicity" relationship showed that after the mice were given different extracts of Cassiae Semen, the serum indexes (ALT, AST, Cr, BUN and TNF-α) in mice reached the peak at 1~2 h after treatment, and 48 h began to recover. The "dose- toxicity" relationship showed that: after single intragastric administration of alcohol extracted components(79.80~26.84)g·kg^-1, water extracted components(57.45 ~23.53)g·kg^-1, all-components 15.00 g·kg^-1 can cause obvious damage in liver and kidney, the serum ALT, AST, BUN, Cr, TNF- levels were significantly increased, which was respectively equal to (387.4~130.3) times, (294.0~120.5) times and 140.0 times of the clinical daily dosage in 70 kg humans. Conclusion Alcohol extracted components and water extracted components which were gavaged with different dose to mice can cause hepatotoxicity and nephrotoxicity and there was a certain relationship of "dose-time-toxicity". However the all-components was no dose-dependent relationship, may be related to the higher concentration of drugs, affecting intestinal absorption.

Key words: Cassiae Semen, different extracts, "dose-time-toxicity" relationship