Rapid Analysis of Multi-indicators in Ginkgo Biloba by Near Infrared Spectroscopy

Abstract

Abstract: ObjectiveTo determine the index ingredient rapidly in Ginkgo biloba with near infrared (NIR) spectroscopy. MethodsHPLC method was used to determine the content of total flavonoids (quercetin, kaempferol and isorhamnetin) in Ginkgo biloba leaves, and near infrared (NIR) spectroscopy was applied to quantitative analysis of the content of flavones in Ginkgo biloba and peak area ratio of quercetin and kaempferol with PLS regression model. ResultsThe correlation coefficient R was 0.9443 and 0.8963 respectively. The root mean square error of cross validation RMSECV was 0.104 and 0.149 respectively. The root mean square error of prediction RMSEP is 0.082 and 0.206 respectively. The root square error of prediction RSEP (%) is 8.69 and 7.29 respectively. ConclusionThe results are good and the method can be applied to the quantitative analysis of index ingredients of Ginkgo biloba.

Key words: near-infrared spectroscopy, rapid analysis, Ginkgo biloba, PLS

CLC Number:

R917

XIANG Yan-hua, SUN Shu-na, WANG Lei, GENG Shu, CHEN Yong. Rapid Analysis of Multi-indicators in Ginkgo Biloba by Near Infrared Spectroscopy[J]. Chinese Journal of Pharmacovigilance, 2015, 12(12): 744-747.

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