Abstract: ObjectiveTo research on the therapeutic index(TI) and the safety factor(SF) of different components of Evodia Fructus when playing analgestic effect. MethodsTo make research on the acute toxicity of different components in accordance with classical acute toxicity test methods; to make research on the analgestic effect of different components in accordance with the classical hot plate method; calculating the LD50, LD5, ED50 and ED95 using Bliss method, to calculate the TI and SF of analgestis effect of different components of Evodia Fructus. ResultsThe LD50 of volatile oil in Evodia Fructus was 2.6995 mL ·kg-1 ·d-1, and the LD5 of volatile oil was 1.9853 mL ·kg-1 ·d-1; the ED50 of volatile oil in Evodia Fructus was 0.4625 mL ·kg-1 ·d-1, and the ED95 of volatile oil was 0.9996 mL ·kg-1 ·d-1; TI was 5.837and SF was 1.986. The water extracts of Evodia Fructus are unable to make LD50, MTD results calculated in accordance with crude drug content was 80.0 g ·kg-1 ·d-1, the ED50 was 1.3515 g ·kg-1 ·d-1, and the ED95 was 4.3435 g ·kg-1 ·d-1. ConclusionBoth the water extracts and volatile oil had certain analgestic effect and had certain dose-efficacy relationship, and the safety scope of water extracts was bigger than volatile oil.

Key words: Evodia Fructus, analgestic effect, safety scope, therapeutic index, safety factor