Abstract: Objective To build a scientific and reasonable way to detect the content of histamine in thymus, in order to control the quality of raw thymus ultimately. Methods We used dansyl chloride pre -column derive, HPLC -UV method, C18 (4.6mm×250mm,5μm) chromatographic column acetonitrile -water asa mobile phase, the flow rate 1.0mL·min ^-1, wavelength 254nm while the gradient elution method was used to detect histamine. Results In the range of 1.0~50μg·mL^-1, the histamine concentration is linear correlation to the peak area, with the correlation coefficient 0.9979. Sample loading recovery is between 85% and 110% , with RSD%约10%. Conclusion Using this way to detect the histamine content in thymus is simple, quick, accurate, sensitive, and easy to repeat. This method can be used as quality control of raw.

Key words: histamine, dansyl chloride pre-column derive, thymus