Abstract: Objective By comparing the acute toxicity of mice, to explore the enrichment of different purification process on the total Bupleurum saikosaponin which is the mainly toxical substance basis of Bupleurum. Methods Make the comparing study on mice's acute toxicity of the crude extracts of total Bupleurum saikosaponin, the water elution of total Bupleurum saikosaponin and the alcohol elution of toatal Bupleurum saikosaponin in accordance with classical acute toxicitytest Methods. Results Caculated by total saikosaponin, the LD₁₅ of the crude of total Bu-pleurum saikosaponin is 0.3g·kg^-1,and the MTD is 0.48 g·kg^-1; the LD₁₀ of the water elution of total Bupleurum saikosaponin is 0.83g·kg^-1,and the MTD is 1.324g·kg^-1; the LD₁₀₀ of the alcohol elution of total Bupleurum saikosaponin is 12.4g·kg^-1. Conclusion The enrichment of different purification process on the total Bupleurum saikosaponin: the alcohol elution of toatal Bupleurum saikosaponin > the water elution of total Bupleurum saikosaponin > the crude extracts of total Bupleurum saikosaponin;the acute toxicity of different purification process on the total Bupleurum saikosaponin: the alcohol elution of toatal Bupleurum saikosaponin > the crude extracts of total Bupleurum saikosaponin> the water elution of total Bupleurum saikosaponin, so we determine that the best refining process of enriching the total Bupleurum saikosaponin is alcohol elution process.

Key words: the crude extracts of total Bupleurum saikosaponin, the alcohol elution, the water elution, mice, acute toxicity