Abstract: Objective To investigate the transfer behavior of pesticide residues in chrysanthemum morifolium under different processing, explore the effects of processing conditions and physicochemical properties of pesticides on the transfer rate, and conduct risk assessment based on the transfer situation of pesticide residues, so as to provide reference for the limit standard of pesticide residues in Chrysanthemum morifolium and the potential risks in the process of using Chrysanthemum morifolium. Methods Through the screening and detection of pesticide residues in chrysanthemum samples, the samples containing high detected pesticide residues were selected as the research objects, and the processing conditions such as water temperature, water boiling and ethanol reflux extraction were systematically studied. The pesticides in the processed samples were determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS), and point assessment method was used to assess the risk of contaminated chrysanthemum and processed products. Results The transfer rate of 15 pesticide residues with high detection rates in chrysanthemum were all transferred to varying degrees during processing, the transfer rates of pesticides during water immersion and boiling were positively correlated with temperature and time, but decreased with increasing processing times; The transfer rate of residual pesticides during ethanol extraction was above 50%; The acute risk quotients of pesticide residues in processed samples were all lower than 5%, indicating a low potential risk. Conclusion The study on the transfer behavior of pesticide residues during the processing of chrysanthemum provides a certain scientific basis for the formulation of the maximum residue standard and residue risk assessment of pesticide in chrysanthemum morifolium.

Key words: chrysanthemum morifolium, pesticide residue, transfer, risk assessment, processing, water immersion, water boiling, ethanol reflux extraction