Study on the incompatibility of Veratrum nigrum Linn. and Sophora flavescens Ait. based on UPLC-QTOF/MS

doi:10.19803/j.1672-8629.20220590

Abstract

Abstract: Objective To investigate the changes of toxic compounds in the co-decoction of Sophora flavescens Ait. and Veratrum nigrum Linn. in order to provide data for studies on their incompatibility. Methods UPLC-QTOF/MS was used to detect the co-decoction and single decoction related to the incompatibility Sophora flavescens Ait. and Veratrum nigrum Linn.. The differences in characteristic compounds between the two groups were analyzed via principal component analysis (PCA), orthogonal partial least squares discriminant (OPLS-DA) and variable importance projection (VIP). The potential characteristic compounds were quickly identified by means of mass spectrometry database search. Results The contents of veratrum steroid alkaloids (VSA) derived from Veratrum nigrum Linn. increased significantly in the co-decoction of Sophora flavescens Ait. and Veratrum nigrum Linn.. A comparison between the two groups showed that there were significant differences in eight chemical components. The contents of veratrosine (peak 3), veralomine (peak 7), pseudojervine (peak 2), veratramine (peak 6), yibeissine (peak 4), 3-veratroyl- zygadenine (peak 9), verazine (peak 11) increased significantly, all of which might be potential chemical markers of toxicity and side effects caused by their compatibility. Conclusion The co-decoction of Sophora flavescens Ait. and Veratrum nigrum Linn. can promote the dissolution of VSA. The incompatibility of Sophora flavescens Ait. and Veratrum nigrum Linn. may be closely related to the content increase of several toxic components. The 10∶1 ratio of the co-decoction can be considered to underlie the contraindication of the incompatibility of Sophora flavescens Ait and Veratrum nigrum Linn..

Key words: Veratrum nigrum Linn., Sophora flavescens Ait., incompatibility, UPLC-QTOF/MS

CLC Number:

R969.2

WANG Chao, WANG Yuguang, MA Zengchun, GAO Yue. Study on the incompatibility of Veratrum nigrum Linn. and Sophora flavescens Ait. based on UPLC-QTOF/MS[J]. Chinese Journal of Pharmacovigilance, 2023, 20(2): 163-167.

References

[1] GAO Y, LI C, LIANG AH, et al.Compatibility for toxicity attenuation of toxic traditional Chinese medicine: a review and strategies[J]. China Journal of Chinese Materia Medica(中国中药杂志), 2022, 47(8): 1989-1994.
[2] JING MQ, ZHANG HP, ZHAO M, et al.Study on the incompatibility mechanism in the combination of Sophora flavescens and Veratrum nigrum by HPLC[J]. Journal of Shenyang Pharmaceutical University(沈阳药科大学学报), 2018, 35(8): 632-638.
[3] GUO YS, XU W, ZHONG GS, et al.Proper and improper condition analysis of ginseng and resveratrolanti-drug combtioination based on the experimental study (中华中医药学会2014第七次临床中药学术研讨会论文集)[C]. Proceedings of the 7th Symposium on Clinical Chinese Medicine, China Association of Traditional Chinese Medicine, 2014.
[4] YANG JB, WANG Q, WANG XT, et al.Difference analysis of chemical composition of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata based on plant metabonomics[J].Chinese Journal of Pharmacovigilance(中国药物警戒), 2022, 19(6): 615-619.
[5] CHEN J, NIE LX, HU XR, et al.Differences of chemical compounds between Glycyrrhiza uralensis Fisch. and Glycyrrhiza glabra L. by UHPLC-Q-TOF-MS/MS[J]. Chinese Journal of Pharmacovigilance(中国药物警戒), 2022, 19(9): 954-958.
[6] WANG B.Studies on the chemical constituents of Veratrum nigrum Linn.[D]. Shanghai:The Second Military Medical University, 2007.
[7] JIA XH, WANG W, GAO MY, et al.Chemical component analysis of Veratrum by UPLC-Q-TOF-MS/MS[J]. Journal of Tianjin University of Traditional Chinese Medicine(天津中医药大学学报), 2021, 40(1): 104-111.
[8] MA YH, ZHANG HF, YU H, et al.Study on the“increase toxicity and decrease effect”of the compatibility of Veratrum nigrum Linn. and Radix Adenophorae[J].Shizhen Guoyi Guoyao(时珍国医国药), 2021, 32(7): 1613-1615.
[9] TANG J, LI HL, HUANG HQ, et al.Progress in the research on chemical constituents of veratrum plants[J]. Progress in Pharmaceutical Sciences(药学进展), 2006, 30(5): 206-212.
[10] GUO JJ, BAO WGQQG, HA SNBQ, et al.Quality differences between Mongolian and traditional Chinese medicine VeratriNigri Radix et Rhizoma by HPLC fingerprint and acute toxicity[J].Chinese Traditional and Herbal Drugs(中草药), 2017, 48(15): 3175-3181.
[11] FAN YQ, ZHAO LL, HUANG XH, et al.UPLC-MS/MS method for the simultaneous quantification of eight compounds in rat plasma and its application to a pharmacokinetic study after oral administration of veratrum (Veratrum nigrum L.) extract[J]. BioMed Res Int, 2020, 33(5): 536-539.
[12] TALDAEV A, TEREKHOV RP, MELNIK EV, et al.Insights into the cardiotoxic effects of veratrum lobelianum alkaloids: pilot study[J]. Toxins, 2022, 14(7): 490-501.
[13] ZHANG B, DONG X, LOU ZY.UHPLC-Q-TOF/MS in rapid separation and identification of chemical components in Veratrum nigrum L.[J]. Academic Journal of Second Military Medical University(第二军医大学学报), 2012, 33(5): 536-539.
[14] CHANDLER CM, MCDOUGAL OM.Medicinal history of North American Veratrum[J]. Phytochem Rev, 2014, 13(3): 671-694.
[15] CHENG MH, RAO GX.Research progress on chemical constituents and pharmacological activities of plants from Veratrum[J]. Chinese Traditional and Herbal Drugs(中草药), 2021, 52(18): 5758-5774.
[16] WANG LS, LI W, LIU YH.Hypotensive effect and toxicology of total alkaloids and veratramine from roots and rhizomes of Veratrum nigrum L. in spontaneously hypertensive rats[J].Pharmazie, 2008, 63(8): 606-610.
[17] CONG Y, GUO JG, TANG Z, et al.Metabolism study of veratramine associated with neurotoxicity by using HPLC-MSⁿ[J].J Chromatogr Sci, 2015, 53(7): 1092-1099.
[18] ZHAO TY, ZHANG XM, LIN ZJ, et al.Chinese materia medica for emetic: Safety analysis and pharmacovigilance considerations[J]. China Journal of Traditional Chinese Medicine and Pharmacy(中华中医药杂志), 2016, 31(6): 2230-2234.
[19] WANG JH, CONG Y, CAO YL.Study on differentiates of the contents of veratrosine and toxicity in processed and crude dried roots and rhizomes of Veratrum nigrum L.[J]. Journal of Henan University Medical Science(河南大学学报医学版), 2007, 26(4): 1-5.
[20] ZHANG MZ, GAO LJ, XU SF, et al.Advances in studies on steroidal alkaloids and their pharmacological activities in genus Veratrum[J]. China Journal of Chinese Materia Medica(中国中药杂志), 2020, 45(21): 5129-5142.
[21] ZHOU JM, WANG YG, CHEN ZW, et al.Modulation of activities and mRNA expression of cytochrome P450 isoenzymes in rat liver by Sophora flaveacens and coadministration with Veratrum nigrum[J].China Journal of Chinese Materia Medica(中国中药杂志), 2010, 35(14): 1845-1849.
[22] ZHANG XX.Study on the interaction and pharmacological effects of Radix paeoniae, Salvia miltiorrhiza. and Veratrum nigrum[D]. Beijing: Academy of Military Medical Sciences, 2015.