Difference analysis of chemical composition of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata based on plant metabonomics

doi:10.19803/j.1672-8629.2022.06.07

Abstract

Abstract: Objective To explore the chemical differences between Polygoni Multiflori Radix (PMR) and Polygoni Multiflori Radix Preparata (PMRP) with the help of plant metabonomics in order to clarify the regularity of chemical composition change before and after processing and the toxicity-attenuating material basis. Methods An ultra-performance liquid chromatography-quadrupole/time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) based metabolomics method was selected to analysis the composition of PMR and PMRP. Principal component analysis (PCA) method was used to observe the overall distribution trend of samples. Statistical analysis techniques were used to screen the differential compounds in the volcano plot. By comparing Agilent Natural Product Personal Compound Database and Library (PCDL), the chemical compositions were preliminarily identified. Results There were significant differences between PMR and PMRP. 45 differential compounds were tentatively identified and their contents were significantly decreased after processing. Conclusion There were significantly difference compounds between PMR and PMRP, especially for anthraquinone (mono and binuclear anthraquinone) and stilbene glycosides, which might be the main material basis for attenuating toxicity of Polygoni multiflorum Thunb.

Key words: Polygoni Multiflori Radix, Polygonum Multiflori Radix Preparata, metabonomics, principal component analysis, Volcano plot, difference compounds, anthrones, stilbene glycosides

CLC Number:

R917
R961

YANG Jianbo, Wang Qi, WANG Xueting, CHENG Xianlong, WANG Ying, GAO Huiyu, SONG Yunfei, WEI Feng, MA Shuangcheng. Difference analysis of chemical composition of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata based on plant metabonomics[J]. Chinese Journal of Pharmacovigilance, 2022, 19(6): 615-619.

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