Influence of miR-205-5p targeting eIF4E on proliferation, invasion and epithelial-mesenchymal transition of MCF-7 cells

doi:10.19803/j.1672-8629.2022.03.06

Abstract

Abstract: Objective To investigate the influence of micro ribonucleic acid-205-5p (miR-205-5p) targeting eukaryotic initiation factor 4E (eIF4E) on the proliferation, invasion and epithelial-mesenchymal transition of human breast cancer MCF-7 cells so as to provide reference for the development of new targeted therapeutic drugs against mammary cancer (MC). Methods MCF-7 cells were cultured, and miR-205-5p was transfected into mimic-NC and miR-205-5p mimic to verify the targeting relationship between miR-205-5p and eIF4E. Meanwhile, the cultured MCF-7 cells were divided into the control group (normally cultured MCF-7 cells), mimic-NC group, pcDNA group, miR-205-5p mimic group, miR-205-5p group, eIF4E-pcDNA group, and miR-205-5p+eIF4E-pcDNA group. The effect of miR-205-5p targeting eIF4E on the proliferation, migration and epithelial-mesenchymal transition of MCF-7 cells was analyzed. Results The relative expression level of miR-205-5p was significantly higher in the miR-205-5p mimic group than in the control group and mimic-NC group, while the mRNA expression level of eIF4E was significantly lower (P<0.05). The luciferase activity (R/F ratio) of eIF4E wild-type and mimic positive group was lower. The relative expressions of eIF4E protein and mRNA were significantly higher in the eIF4E-pcDNA group than in the control group and pcDNA group (P<0.05). The positive rate of MCF-7 cells, colony distribution rate, Ki67 and PCNA protein expression levels in the miR-205-5p group were significantly lower than those in the control group, eIF4E-pcDNA group, and miR-205-5p+eIF4E-pcDNA group (P<0.05). The positive rate and number of aggressive cells in the miR-205-5p group were significantly lower or smaller than those in the control group, miR-205-5p+eIF4E-pcDNA group, and eIF4E-pcDNA group (P<0.05), but the number of aggressive cells was the largest in eIF4E-pcDNA group. The positive expression rates and protein expression levels of the epithelial mesenchymal markers E-Cadherin, N-Cadherin, and Vimentin of MCF-7 cells were significantly lower in the miR-205-5p group than in the control group, miR-205-5p+eIF4E-pcDNA group, and eIF4E-pcDNA group (P<0.05), but significantly higher in the eIF4E-pcDNA group than in the other groups (P<0.05). Conclusion miR-205-5p can effectively inhibit the proliferation, invasion and epithelial-mesenchymal transition of human breast cancer MCF-7 cells by negatively targeting eIF4E, which is expected to provide reference for the development of new gene-targeted therapeutic drugs against MC.

Key words: miR-205-5p, eIF4E, human breast cancer cell, MCF-7

CLC Number:

R979.1

ZHANG Naxian, LIU Liu, LI Gang, LIU Linrui, LI Yuanying. Influence of miR-205-5p targeting eIF4E on proliferation, invasion and epithelial-mesenchymal transition of MCF-7 cells[J]. Chinese Journal of Pharmacovigilance, 2022, 19(3): 259-264.

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