Abstract: Objective To explore the therapeutic effect of Reduning inhalation solution against the viral pneumonia model of mice infected with influenza A/PR/8/H1N1 so as to provide reference for clinical application.Methods Laboratory animals were randomly divided into eight groups according to their body weight: control group, model group, ribavirin group, Reduning injection group, and four Reduning aerosol inhalation groups including the 0.23 g crude drug/kg high-dose group (20 min), 0.12 g crude drug/kg middle-dose group (10 min) , 0.06 g crude drug/kg low-dose group (5 min) and 0.03 g crude drug/kg very-low-dose group (2.5 min) . A pneumonia model of mice was established via intranasal infection with influenza A/PR/8/H1N1. The effectiveness of Reduning inhalation solution was evaluated by detecting the lung index, lung index inhibition rate, pathological changes, viral load, as well as the production of IL-6 and TNF-α in lung tissue. Results All the four treatments with Reduning inhalation solution could reduce the lung index of mice, alleviate the pathological damage to lung tissue, and reduce the viral load and the content of IL-6 and TNF-α in lung tissue to different extents.Conclusion Aerosol inhalation of Reduning has a significant therapeutic effect against the pneumonia model infected with influenza A/PR/8/H1N1.

Key words: influenza A/PR/8/H1N1, virus-associated pneumonia, Reduning, inhalation solution, viral load, inflammatory cytokine