中国药物警戒 ›› 2025, Vol. 22 ›› Issue (6): 627-632.
DOI: 10.19803/j.1672-8629.20250215

• 基础与临床研究 • 上一篇    下一篇

痰热清雾化吸入对慢阻肺动物的药效评价及氧化应激线粒体损伤机制研究

符蕤, 罗倩, 彭博, 陈韡亚, 魏晓露, 陈腾飞, 宋玲, 高云航, 张广平#, 侯红平*   

  1. 中国中医科学院中药研究所,道地药材品质保障与资源持续利用全国重点实验室,北京 100700
  • 收稿日期:2025-04-10 发布日期:2025-06-18
  • 通讯作者: *侯红平,女,副研究员,慢性疾病。E-mail: hphou@icmm.ac.cn;#为共同通信作者。
  • 作者简介:符蕤,女,在读硕士,慢性疾病。
  • 基金资助:
    国家自然科学基金资助项目（82104502）; 中央级公益性科研院所基本科研业务费专项基金资助项目（ZXKT23006、ZZ13-YQ-060、ZXKT21020）; 中国中医科学院科技创新工程项目资助（CI2023E002）

Efficacy of Tanreqing Aerosol Inhalation against Animals with Chronic Obstructive Pulmonary Disease and Mechanisms for Oxidative Stress and Mitochondrial Injury

FU Rui, LUO Qian, PENG Bo, CHEN Weiya, WEI Xiaolu, CHEN Tengfei, SONG Ling, GAO Yunhang, ZHANG Guangping#, HOU Hongping*   

  1. State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
  • Received:2025-04-10 Published:2025-06-18

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摘要： 目的 探讨痰热清雾化吸入对慢阻肺模型动物药效及氧化应激和线粒体损伤的作用。方法 SPF级Wistar大鼠随机分为对照组,模型组,阳性组和高、中、低给药组（0.12、0.06、0.03 mL·kg-1）。除对照组外,其余各组进行慢阻肺模型的制作,阳性组雾化吸入布地奈德混悬液20 min,给药组雾化吸入痰热清20、10、5 min。1个月后,观察动物的肺功能和组织病理学,同时检测氧化应激和线粒体损伤的相关指标。结果 模型组大鼠自主活动减少,肺顺应性降低,气道阻力升高,活性氧（ROS）和丙二醛（MDA）升高,超氧化物歧化酶（SOD）和总抗氧化能力（T-AOC）降低,线粒体通透性转换孔（mPTP）和腺嘌呤核苷三磷酸（ATP）水平下降,细胞色素c（Cytc）释放增多。而阳性组和痰热清高、中剂量的大鼠临床症状有所缓解,气道阻力降低,肺顺应性升高。进一步研究发现,其ROS和MDA水平有所降低,SOD和T-AOC显著升高,P<0.01。mPTP和ATP水平显著升高,Cytc水平显著降低,P<0.01或P<0.001。结论 痰热清雾化吸入通过干预氧化应激和线粒体损伤的水平起到缓解慢阻肺症状的作用,为中药治疗慢阻肺提供参考。

关键词: 痰热清, 雾化吸入, 慢阻肺, 氧化应激, 线粒体损伤, 大鼠

Abstract: Objective To study the effects of aerosol inhalation of Tanreqing on animal models of chronic obstructive pulmonary disease (COPD) and explore the mechanisms for oxidative stress and mitochondrial damage. Methods SPF Wistar rats were randomly divided into the control group, model group, positive group and high-, medium- and low-dose medication groups（0.12、0.06、0.03 mL·kg-1）. COPD models were established in each group except the control group. The positive group inhaled budesonide suspension for 20 min while the medication groups inhaled Tanreqing for 20, 10 and 5 min, respectively. After one month, the lung function and histopathology of the animals were observed, and related indexes of oxidative stress and mitochondrial damage were detected. Results In the model group, autonomic activities decreased, so did lung compliance, the airway resistance, ROS and MDA increased, SOD and T-AOC decreased, so did mPTP and ATP levels, but Cytc release increased. However, the clinical symptoms of rats in the positive group and in the high-dose and medium-dose groups were relieved, the airway resistance decreased, but lung compliance improved. The levels of ROS and MDA decreased, while those of SOD and T-AOC increased significantly, P<0.01, so did the levels of mPTP and ATP, but the levels of Cytc decreased significantly, P<0.01 or P<0.001. Conclusion Aerosol inhalation of Tanreqing could relieve the symptoms of COPD by intervening in oxidative stress and mitochondrial damage, which provides a reference for treatment of COPD with traditional Chinese medicine.

Key words: Tanreqing, Aerosol Inhalation, Chronic Obstructive Pulmonary Disease, Oxidative Stress, Mitochondrial Damage, Rats

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