中国药物警戒 ›› 2012, Vol. 9 ›› Issue (12): 705-706.

• 基础及临床研究 •    下一篇

高效液相色谱法测定小鼠胰腺组织中头孢匹胺浓度

蒋军, 夏宗玲, 王明丽   

  1. 江苏省常州市第一人民医院,江苏 常州 213003
  • 收稿日期:2012-09-10 出版日期:2012-12-10 发布日期:2015-08-07
  • 作者简介:蒋军,男,本科,主管药师,医院药学和临床药学。
  • 基金资助:
    常州市科技局指导性项目（常州四药临床药学研究基金）（基金编号：CY20110022）

Determination of Cefpiramide Concentration in Pancreatic Tissue of Mice by RP-HPLC

JIANG Jun, XIA Zong-ling, WANG Ming-li   

  1. Changzhou First People's Hospital, Jiangsu Changzhou 213003, China
  • Received:2012-09-10 Online:2012-12-10 Published:2015-08-07

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摘要： 目的建立高效液相色谱法（HPLC）测定小鼠胰腺组织中头孢匹胺浓度的方法。方法以DISCOVERY ODS C18柱（5 m,250×4.6 mm）为色谱柱,甲醇 三乙胺醋酸 水（27 0.2 72.8,v/v）为流动相,流速1.0 mL ·min-1,柱温30℃,紫外检测波长254nm。结果小鼠胰腺组织中的内源性物质不干扰头孢匹胺的测定,头孢匹胺在0.1~500.0μg ·mL-1浓度范围内峰面积与浓度线性关系良好（r=0.999 8）,最低检测限为0.1μg ·mL-1。平均回收率为（97.18±0.74）%,高、中、低3种浓度的日内差（RSD）<5%,日间差（RSD）<10%。结论本方法简便、快速、准确,重现性好,可应用于头孢匹胺组织内药动学研究。

关键词: 头孢匹胺, 高效液相色谱法, 胰腺

Abstract: ObjectiveTo establish a reversed-phase HPLC(RP-HPLC) technique for the quantification of the cefpiramide(CPM) concentration in pancreatic tissue. MethodsThe following RP-HPLC conditions were set up DISCOVERY ODS C18 column(5 m, 250 mm×4.6 mm) as stationary phase; a mixture of methanol, triethanolamine and water(27-0.2-72.8) at a flow rate of 1.0 mL ·min-1 as mobile phase; UV detection at 254 nm. ResultsCPM concentration of pancreatic tissue showed a linear plot in the range of 0.1~500.0μg ·mL-1(r=0.999 8). The minimal quantitative concentration was 0.1μg ·mL-1. The mean recovery at the quantitative concentrations of CPM was (97.18±0.74)%. The coefficients of variation for within-day performance were found to be less than 5%, and between-day performance less than 10%. ConclusionThe RP-HPLC is simple and sensitive to quantify cefpiramide concentration of pancreatic tissue, and may be used to monitor the PK parameters in tissues.

Key words: Cefpiramide, HPLC, pancreas