2025年4月7日 星期一

中国药物警戒 ›› 2024, Vol. 21 ›› Issue (6): 655-659.
DOI: 10.19803/j.1672-8629.20230300

• 基础与临床研究 • 上一篇    下一篇

佛芍颗粒对小鼠肠道菌群及乳糖酶活性的调控作用研究

李舒冉1, 郭姗姗1, 赵荣华1, 包蕾1, 孙静1, 耿子涵1, 鲍岩岩1, 庞博1, 陈梦苹1, 吕耀中2,3, 王振中2,3, 崔晓兰1,*, 王红梅2,3#   

  1. 1中国中医科学院中药研究所生物安全实验室,北京100700;
    2中药制药过程控制与智能制造技术全国重点实验室,江苏 连云港 222000;
    3江苏康缘药业股份有限公司,江苏 连云港 222000
  • 收稿日期:2023-05-11 出版日期:2024-06-15 发布日期:2024-06-18
  • 通讯作者: *崔晓兰,女,博士,研究员·博导,中药防治感染性疾病机制研究。E-mail: xlcui@icmm.ac.cn; #为共同通信作者。
  • 作者简介:李舒冉,女,博士,助理研究员,中药防治感染性疾病机制研究。
  • 基金资助:
    国家自然科学基金资助项目（82141206）; 中央级公益性科研院所基本科研业务费专项资金资助（ZZ15-YQ-035; ZXKT21031）; 中国中医科学院科技创新项目（CI2021B015）

Roles of Foshao granules in regulating gut microbiota and lactase activity in mice

LI Shuran1, GUO Shanshan1, ZHAO Ronghua1, BAO Lei1, SUN Jing1, GENG Zihan1, BAO Yanyan1, PANG Bo1, CHEN Mengping1, LYU Yaozhong2,3, WANG Zhenzhong2,3, CUI Xiaolan1,*, WANG Hongmei2,3#   

  1. 1Biosecurity Laboratory, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;
    2National Key Laboratory of Process Control and Intelligent Manufacturing Technology of Traditional Chinese Medicine Pharmaceuticals, Lianyungang Jiangsu 222000, China;
    3Jiangsu Kangyuan Pharmaceutical Co., Ltd, Lianyungang Jiangsu 222000, China
  • Received:2023-05-11 Online:2024-06-15 Published:2024-06-18

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摘要： 目的 明确佛芍颗粒对小鼠肠道菌群失调及乳糖酶活性的调节作用,为临床应用提供基础研究数据支持。方法 实验小鼠按体质量随机分为正常组,模型组,吗丁啉组（12 mg·kg-1）,四逆散组（3 g·kg-1）,佛芍颗粒高剂量组（48 g生药·kg-1）、佛芍颗粒中剂量组（24 g生药·kg-1）、佛芍颗粒低剂量组（12 g生药·kg-1）,共7组,每组10只,雌雄各半。采用头孢拉定胶囊和硫酸庆大霉素（2∶1）混合物灌胃诱导肠道菌群失调模型小鼠,通过检测小鼠肠道总厌氧菌、乳酸杆菌、双歧杆菌、大肠杆菌数量及结肠黏膜乳糖酶活性水平,综合评价佛芍颗粒对菌群失调和乳糖酶活性的调控作用。结果 佛芍颗粒可以明显增加总厌氧菌（P<0.01）、乳酸杆菌（P<0.01）、双歧杆菌（P<0.01）及大肠杆菌（P<0.01）的数量,并提高结肠黏膜乳糖酶活性。结论 佛芍颗粒可以改善头孢拉定胶囊和硫酸庆大霉素诱导的菌群失调和乳糖酶活性降低,为佛芍颗粒的临床应用提供参考。

关键词: 佛芍颗粒, 头孢拉定, 庆大霉素, 菌群失调, 乳糖酶活性, 肠道, 小鼠

Abstract: Objective To study the effect of Foshao granules on the gut microbiota and lactase activity of mice. Methods Experimental rats were randomly divided into seven groups according to their body weight: the normal control group, model control group, domperidone control group (12 mg·kg-1), Sini powder control group (3 g·kg-1), Foshao granules high dosage group (48 g crude drug·kg-1), medium dosage group (24 g crude drug·kg-1), and low dosage group (12 g crude drug·kg-1), with 10 mice in each. A model of flora imbalance mice was induced by a mixture of cefradine capsules and gentamycin sulfate (2 : 1). By measuring the number of total anaerobic bacteria, Lactobacillus, bifidobacteria, Escherichia coli, the extent to which Foshao granules regulated the gut microbiota and lactase activity of mice was evaluated. Results Foshao granules could significantly increase the number of total anaerobic bacteria (P <0.01), Lactobacillus (P <0.01), bifidobacteria (P <0.01) and Escherichia coli(P <0.01), and elevated the colonic mucosal lactase activity. Conclusion Foshao granules can improve flora imbalance and the reduction of lactase activity caused by cefradine and gentamicin sulphate. This study is expected to provide data for clinical applications of Foshao granules.

Key words: Foshao granules, cefradine, gentamicin, flora imbalance, lactase activity, intestinal tract, mice

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