中国药物警戒 ›› 2018, Vol. 15 ›› Issue (9): 526-529.

• 垂体肿瘤治疗靶点及药物专栏 • 上一篇    下一篇

量化成像流式法筛选抑制催乳素腺瘤的药物研究

李丹1, 王红云1, 李建华2, 刘潜1, 高华1*   

  1. 1北京市神经外科研究所,北京 100050;
    2山东省滨州市人民医院神经外科,山东 滨州 256610
  • 收稿日期:2018-11-02 修回日期:2018-11-02 出版日期:2018-09-20 发布日期:2018-11-02
  • 通讯作者: 高华,博士,副研究员,脑肿瘤的基础与临床研究。E-mail：huagao@aliyun.com
  • 作者简介:李丹,本科,实验师,中枢神经系统肿瘤药物开发。
  • 基金资助:
    北京市自然科学基金(7162035):Notch通路及抑制剂在生长激素腺瘤发生发展的调控作用及机制的研究;北京市卫计委高层次人才计划-学术骨干(2015-3-040)

Study on Screening Natural Compounds Inhibiting Prolactinomas by Quantitative Imaging Flow Cytometry

LI Dan1, WANG Hongyun1, LI Jianhua2, LIU Qian1, GAO Hua1*   

  1. 1Beijing Neurosurgical Institute, Beijing 100050, China;
    2Department of Neurosurgery, Binzhou People's Hospital, Shandong Binzhou 256610, China
  • Received:2018-11-02 Revised:2018-11-02 Online:2018-09-20 Published:2018-11-02

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摘要： 目的 利用量化成像流式法筛选抑制催乳素腺瘤MMQ细胞生长的天然药物,观察候选药物对MMQ细胞分泌催乳素能力的影响。方法 10种天然化合物处理MMQ细胞24 h,量化成像流式法检测细胞形态和凋亡水平,ELISA法观察细胞培养上清的催乳素含量,Western blot试验观察剪切Caspase 3的水平。结果 利用成像流式法筛选出异硫氰酸烯丙酯、橘皮素和黄腐酚3种天然化合物对MMQ细胞有诱导细胞凋亡、抑制增殖的作用,处理24 h后对照组催乳素为107.3±22.7 pg·mL-1,异硫氰酸烯丙酯组为48.3±13.2 pg·mL-1,橘皮素组为52.7±12.6 pg·mL-1,黄腐酚组为34.7±8.2 pg·mL-1。蛋白印迹试验显示橘皮素和黄腐酚活化caspase 3能力显著（P <0.01）。结论 利用成像流式法进行药物筛选,能够兼顾成像和量化分析特点,异硫氰酸烯丙酯、橘皮素和黄腐酚有作为催乳素腺瘤治疗候选化合物的潜能。

关键词: 成像流式法, 催乳素腺瘤, 药物筛选, 凋亡

Abstract: Objective To screen the natural compounds inhibiting prolactinomas MMQ cells with quantitative imaging flow cytometry, and observe the changes of prolactin levels. Methods Treated with 10 natural compounds to MMQ cells for 24 hours, cell morphology and apoptotic cells were determined by imaging flow cytometry. The medium prolactin level was measured by ELISA method. Cleaved Caspase 3 level was determined by Western blot method. Results Allylisothiocyante, tangeretin and xanthohumol screened from 10 natural compounds could effectively induce the MMQ cells apoptosis. After 24 hours treatment, the prolactin levels reduce to 48.3±13.2, 52.7±12.6 and 34.7±8.2 pg·mL-1, respectively, and 107.3±22.7 pg·mL-1 in control group. Western blot experiment showed the cleaved Caspase 3 level obviously increase after tangeretin and xanthohumol treatment (P<0.01). Conclusion Imaging flow cytometry has the advantage of combination of quantitative data analysis and fluorescent image, and allylisothiocyante, tangeretin and xanthohumol could be candidate drug for the prolactinomas treatment.

Key words: imaging flow cytometry, prolactinomas, drug screening, apoptosis

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