中国药物警戒 ›› 2018, Vol. 15 ›› Issue (9): 523-525.

• 垂体肿瘤治疗靶点及药物专栏 • 上一篇    下一篇

p38MAPK通路特异性抑制剂SB203580对大鼠垂体GH3细胞增殖和分泌的影响

何乐, 龚磊, 李储忠, 王红云, 李丹, 刘潜*   

  1. 北京市神经外科研究所,北京 100050
  • 收稿日期:2018-11-02 修回日期:2018-11-02 出版日期:2018-09-20 发布日期:2018-11-02
  • 通讯作者: 刘潜,女,博士,中枢神经系统肿瘤及分子药理研究。E-mail：liuqian1313@163.com
  • 作者简介:何乐,专科,主管技师,脑肿瘤的分子分类和个体化治疗。
  • 基金资助:
    国家自然科学基金(81502154):EGFL7调控EGFR介导的Akt/MAPK通路促进垂体腺瘤侵袭及机制研究

Effects of p38 Mitogen-activated Protein Kinase Inhibitor SB203580 on Proliferation and Growth Hormone Secretion of Rat Pituitary GH3 Cells

HE Yue, GONG Lei, LI Chuzhong, WANG Hongyun, LI Dan, LIU Qian*   

  1. Beijing Neurosurgical Institute, Beijing 100050, China
  • Received:2018-11-02 Revised:2018-11-02 Online:2018-09-20 Published:2018-11-02

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摘要： 目的 观察p38 MAPK通路特异性抑制剂SB203580对生长激素腺瘤GH3细胞增殖和分泌的影响,以探索p38 MAPK通路作为生长激素腺瘤潜在治疗靶点的可行性。方法 MTT实验检测不同浓度SB203580处理生长激素腺瘤GH3细胞24、48和72 h后细胞增殖活力。不同浓度的SB203580处理生长激素腺瘤GH3细胞72 h后经Annexin V-FITC/PI双染,流式细胞仪分析细胞凋亡的情况。ELISA检测不同浓度的SB203580处理生长激素腺瘤GH3细胞72 h后细胞上清中生长激素的分泌水平。结果 SB203580能够有效抑制生长激素腺瘤GH3细胞的增殖（P <0.05）,并呈现良好的时间依赖性与浓度依赖性。不同浓度的SB203580能够显著诱导生长激素腺瘤GH3细胞凋亡（P <0.05）。SB203580处理泌生长激素腺瘤GH3细胞可显著降低生长激素水平（P <0.05）,呈现良好的浓度依赖性。结论 p38 MAPK通路特异性抑制剂SB203580可抑制生长激素腺瘤GH3增殖和生长激素分泌,并能促进细胞凋亡。

关键词: MAPK信号通路, SB203580, 抑制剂, 生长激素腺瘤, GH3细胞

Abstract: Objective To observe the effects of p38 mitogen-activated protein kinase inhibitor SB203580 on proliferation and growth hormone secretion of rat pituitary GH3 cells and explore the p38 MAPK pathway as a potential therapy target for growth homone adenoma. Methods After treated with different concentrations of SB203580 for 24, 48, 72 h, the proliferation of rat pituitary GH3 cells was determined by MTT. The apoptosis levels were measured by Annexin V/PI. And the expression of growth hormone levels in the supernatant of GH3 cells treated with increasing concentrations of SB203580 for 72 h were measured by ELISA. Results SB203580 caused significant cytotoxicity in GH3 cells with a concentration- and time-dependent manner. Treatment with SB203580 induced GH3 cells apoptosis in a concentration-dependent manner (P <0.05). In addition, the level of growth hormone was significantly decreased in the supernatant of GH3 cells after exposure to SB203580 (P <0.05). Conclusion p38 mitogen-activated protein kinase inhibitor SB203580 may inhibit the proliferation and growth hormone secretion of rat pituitary GH3 cells.

Key words: MAPK cell signal, SB203580, inhibitor, growth homone adenoma, GH3 cell line

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