中国药物警戒 ›› 2016, Vol. 13 ›› Issue (11): 670-672.

• 质量与工艺研究 • 上一篇    下一篇

HPLC法测定麝香追风膏中盐酸麻黄碱含量

蒋小文   

  1. 南京市食品药品监督检验院,江苏 南京 210038
  • 收稿日期:2016-12-05 修回日期:2016-12-05 出版日期:2016-11-20 发布日期:2016-12-05
  • 作者简介:蒋小文,女,硕士,主管中药师,中药饮片及中成药质量检验与研究。

HPLC Determination of Ephedrine Hydrochloride in Shexiangzhuifeng Ointmen

JIANG Xiao-wen   

  1. Nanjing Food and Drug Supervision and Inspection Institute, Jiangsu Nanjing 210038,China
  • Received:2016-12-05 Revised:2016-12-05 Online:2016-11-20 Published:2016-12-05

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摘要： 目的 建立高效液相色谱法测定麝香追风膏中盐酸麻黄碱的含量。方法 采用高效液相色谱法,色谱柱为Welch极性乙醚连接苯基柱（5 µm,250 mm×4.6 mm）,流动相为甲醇-0.092%磷酸溶液（含0.02%二正丁胺和0.04%三乙胺）（1.5：98.5）,检测波长210 nm,流速1.0 mL·min-1,进样量10 µL。结果 盐酸麻黄碱在1.66×10-4~0.244μg范围内呈良好的线性关系,r=0.999 9,平均回收率为101.8%,RSD为1.2%。结论 采用本方法 测定麝香追风膏中盐酸麻黄碱含量,准确、方便、专属性强,可用于麝香追风膏的质量控制。

关键词: 麝香追风膏, 高效液相色谱, 盐酸麻黄碱, 含量测定

Abstract: Objective To determine the content of ephedrine hydrochloride in Shexiangzhuifeng ointmen by HPLC. Methods HPLC with Welch polar ether linked phenyl column (5 µm, 150 mm×4.6 mm) was employed by using methyl cyanide-0.092% H3PO4 (0.04% triethylamine and 0.02% dibutylamine) (1.5:98.5) as the mobile phase. The flow rate was 1.0 mL·min-1. The detection wavelength was set at 210 nm. The sample size was 10 µL. Results Ephedrine hydrochloride was in good linear relationship within the rage of 1.66×10-4~0.244 µg, r=0.999 9. The mean recovery rate was 101.8% and RSD was 1.2%. Conclusion The HPLC method established here for analyzing the ephedrine hydrochloride in Shexiangzhuifeng ointmen is accurate, convenient and specific, which can be used for the quality control (QC).

Key words: Shexiangzhuifeng ointmen, HPLC, ephedrine hydrochloride, content determination

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