中国药物警戒 ›› 2016, Vol. 13 ›› Issue (6): 338-340.

• 质量与工艺研究 • 上一篇    下一篇

HPLC测定柴葛清热颗粒中柴胡皂苷a、葛根素和黄芩苷含量

曾俊芬, 鲁建武, 宋金春   

  1. 武汉大学人民医院药学部,湖北 武汉430060
  • 收稿日期:2016-07-22 修回日期:2016-07-22 出版日期:2016-06-20 发布日期:2016-07-22
  • 作者简介:曾俊芬,女,博士,主管药师,中药及天然药物。E-mail：zengjf1979@126.com
  • 基金资助:
    湖北省科技计划项目(2012DCA03001 )

Determination of Saikosaponin a, Paeoniflorin and Baicalin in Chaigeqingre Granula by HPLC

ZENG Jun-fen, LU Jian-wu, SONG Jin-chun   

  1. Department of Pharmacy, Renmin Hospital of Wuhan University, Hubei Wuhan 430060, China
  • Received:2016-07-22 Revised:2016-07-22 Online:2016-06-20 Published:2016-07-22

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摘要： 目的 建立柴葛清热颗粒中柴胡皂苷a、葛根素和黄芩苷含量的测定方法。方法 采用HPLC法,色谱柱为Agilent-C18色谱柱（150 mm×4.6 mm,5 μm）,流动相：0.1%磷酸水溶液(A)-乙腈(B),梯度洗脱（0~12 min,15%~20% B;12~30 min,20%~25% B）,流速为1.0 mL·min-1,检测波长210 nm,柱温30℃,进样量20 μL。结果 胡皂苷a在1.96~31.36 μg·mL-1,葛根素在9.4~150.4 μg·mL-1,黄芩苷1.52~24.32 μg·mL-1范围内线性关系良好。平均回收率分别为97.65%、103.56%、102.83%,RSD分别为1.29%、1.14%、1.20%。结论 该方法准确简便重复性好,可用于柴葛清热颗粒中柴胡皂苷a、葛根素和黄芩苷的含量测定。

关键词: 柴葛清热颗粒, 柴胡皂苷a, 葛根素, 黄芩苷, 高效液相色谱

Abstract: Objective To establish a method for the determination of saikosaponin a, paeoniflorin and baicalin in Chaigeqingre granula by HPLC. Methods The chromatographic column was Agilent-C18(150 mm×4.6 mm, 5 μm), using the gradient mobile phase composed of 0.1% H3PO4 (A) -acetonitrile (B), (0~12 min, 15~20% B; 12~30 min, 20~25% B), the flow rate was 1.0 mL·min-1 and the detection wavelength was 210 nm. The temperature of column was 30℃, and the injection volume was 20 μL．Results The linear range of saikosaponin a, paeoniflorin and baicalin was 1.96~31.36 μg·mL-1, 9.4~150.4 μg·mL-1 and 1.52~24.32 μg·mL-1 with average recovery of 97.65% (RSD=1.29%), 103.56% (RSD=1.14%), 102.83% (RSD=1.20%). Conclusion The method is convenient, accurate and reproducible for the determination of saikosaponin a, paeoniflorin and baicalin in Chaigeqingre granula.

Key words: Chaigeqingre granula, saikosaponin a, paeoniflorin, baicalin, HPLC

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