中国药物警戒 ›› 2010, Vol. 7 ›› Issue (11): 644-645.

• 基础研究 • 上一篇    下一篇

中药桔梗对丝裂霉素诱发小鼠骨髓微核率的影响

狄艳琴1, 管清香2   

  1. 1.解放军第208医院药剂科,吉林长春130062；
    2.吉林大学药学院药剂教研室,吉林长春130021
  • 收稿日期:2010-10-10 修回日期:2016-03-09 出版日期:2010-11-08 发布日期:2016-03-09
  • 通讯作者: 管清香,女,副教授,药剂学研究。E-mail: gqxjldx@126.com
  • 作者简介:狄艳琴,女,主管药师,药品调剂与研究。

Effect of Platycodon Grandiflorum(Jaoq.)A.DC on the Micronuclei Frequencies Induced by Mitomycin in the Bone Marrow Cells of Mice

DI Yan-qin1 ,GUAN Qing-xiang2   

  1. 1.Department of Pharmacy, 208 th Hospital of PLA, JilinChangchun 130062, China;
    2.Department of Pharmacy, School of Pharmaceutical Sciences, Jilin University, JilinChangchun 130021, China
  • Received:2010-10-10 Revised:2016-03-09 Online:2010-11-08 Published:2016-03-09

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摘要： 目的 观察中草药桔梗对丝裂霉素（MMC）诱发小鼠微核的抑制作用。方法 50 只小鼠随机分为阴性对照组（N·S）,阳性对照组（MMC,30mg·kg-1）；桔梗水煎液诱变组（1.0、2.0、4.0 和8.0 g·kg-1,相当于人5、10、20、40 倍临床常用剂量）；桔梗水煎液抗诱变组（MMC 0.4mg·kg-1＋桔梗各剂量组）。每组5 只动物,雌雄兼用。MMC 腹腔注射给药,其他药物均为灌胃给药,抗诱变实验组桔梗和MMC 同时给药,小鼠骨髓嗜多染红细胞微核实验按常规方法进行。结果 桔梗各剂量组所诱导的小鼠微核频率与正常对照组比较,差异无显著性（ >0.05）。桔梗本身不能诱发小鼠微核,并且4.0g·kg-1,8.0g·kg-1 组与丝裂霉素一同给药时,可使丝裂霉素所诱发的小鼠微核率明显降低（ P＜0.05）。结论 桔梗对遗传物质无诱变作用,对小鼠遗传物质具有一定的保护作用。

关键词: 桔梗, 丝裂霉素, 抗诱变

Abstract: Objective To observe the inhibitory effects of Platycodon grandiflorum(Jaoq.) A.DC on micronuclei frequencies induced by MMC in the bone marrow cells of mice. Methods Fifty mice were divided into groups: negative control (N·S), positive control (MMC 0.4 mg·kg-1), Platycodon Grandiflorum(Jaoq.)A.DC mutagenesis groups (1.0、2.0、4.0 and 8.0g·kg-1, which were matched5、10、20、40 clinical commonly used dosage ofa person) Platycodon Grandiflorum(Jaoq.)A.DC antimutagenesis groups ( MMC 30mg·kg -1 + each Platycodon Grandiflorum(Jaoq.)A.DC dosage Platycodon Grandiflorum(Jaoq.)A.DC groups), 5 mice per groups. MMC are given by drug abdominal cavity Injection, other drug are given by perfusing stomach. Platycodon Grandiflorum(Jaoq.)A.DCand MMC are given simultaneously in antimutagenesis groups, oncea day for 5 days successively. At the 5th weekend after the first administration, the mice are killed by cervical vertebra dislocation whose marrow are drawed and sheets are produced in our laboratory method. Results the micro-nuclei frequencies was no significant difference between tested groups and control group(P<0.05), but the frequency of micronucle induced by MMC was reduced by intragastrial perfusion of Platycodon Grandiflorum(Jaoq.)A.DC (4.0g·kg-1, 8.0g·kg-1) and MMC at the same time(约0.01). Conclusion Platycodon Grandiflorum(Jaoq.)A.DC can not induce micronulei formation in the mouse bone marrow cells, but showsa protective effect on genetic materials in mice.

Key words: Platycodon Grandiflorum(Jaoq.)A.DC , mitomycin, antimutagenic agents

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