中国药物警戒 ›› 2010, Vol. 7 ›› Issue (3): 139-141.

• 基础研究 • 上一篇    下一篇

HPLC法同时测定大黄牡丹汤中大黄酸、丹皮酚和芍药苷的含量

刘丽, 尹运涵, 吴健, 李典赛, 赵春杰*   

  1. 沈阳药科大学 药学院,辽宁 沈阳 110016
  • 收稿日期:2016-02-19 修回日期:2016-02-19 出版日期:2010-03-08 发布日期:2016-02-19
  • 通讯作者: 赵春杰,男,教授,中药质量控制技术研究。E-mail：zcjjljj@sina.com
  • 作者简介:刘丽,女,在读硕士研究生,中药质量分析研究。

Simultaneous Determination of Rhein, Paeonol and Paeoniflorin in Decoction of Rheum and Moutan by HPLC

LIU Li, YIN Yun-han, WU Jian, LI Dian-sai, ZHAO Chun-jie*   

  1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China
  • Received:2016-02-19 Revised:2016-02-19 Online:2010-03-08 Published:2016-02-19

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摘要： 目的 建立HPLC法同时测定大黄牡丹汤中大黄酸、丹皮酚和芍药苷的含量。方法 采用Kromasil C18色谱柱（250 mm×4.6 mm,5 m）,流动相：乙腈-体积分数为0.1%的磷酸水溶液梯度洗脱,流速：1.0 mL·min-1,检测波长：230 nm,进样量：10 L。结果 大黄酸的线性为1.61~16.1 mg·L-1（r=0.9997）,平均回收率为98.0%,RSD为0.7%;丹皮酚的线性为1.64~16.4 mg·L-1（r=0.9997）,平均回收率为99.8%,RSD为1.3%;芍药苷的线性为1.54~15.4 mg·L-1（r=0.9996）,平均回收率为100.3%,RSD为1.9%。结论 该方法能更加全面地控制该方的质量,优化后的提取工艺可为今后剂型的改进奠定基础。

关键词: 高效液相色谱法, 大黄牡丹汤, 有效成分

Abstract: Objective To determine 3 components in decoction of Rheum and Moutan by HPLC simultaneously. Methods The seperation of 3 ingerdients was achieved on a Kromasil C18 column(250 mm×4.6 mm,5 m) with gradi-ent elution using solvents of acetonitrile 0.1% phosphoric acid solution. The speed of flowing phase was 1.0 mL·min-1.The detection wavelength was at 230 nm. Results The linear range was 1.61~16.1 mg·L-1 for rhein, 1.64~16.4 mg·L-1 for paeonol and 1.54~15.4 mg·L-1 for paeoniflorin. Average recoveries of samples were all over 98.0%, RSD were all below 2.0%. Conclusion The method was simple, accurate and can be used as assessment about quality of Decoction of Rheum and Moutan.

Key words: High Performance Liquid Chromatography-Fluorescence(HPLC), decoction of Rheum and Moutan, main gredients

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