中国药物警戒 ›› 2010, Vol. 7 ›› Issue (2): 65-69.

• 基础研究 •    下一篇

益母草不同炮制品的小鼠急性毒性实验研究

黄伟1,2, 孙蓉2*, 张作平3   

  1. 1山东省中医药研究院,山东 济南 250014;
    2山东中医药大学,山东 济南 250355;
    3济南杏林生物技术有限公司,山东 济南 250101
  • 收稿日期:2016-02-19 修回日期:2016-02-19 出版日期:2010-02-08 发布日期:2016-02-19
  • 通讯作者: 孙蓉,女,研究员,中药药理与毒理研究。
  • 作者简介:黄伟,男,在读硕士研究生,中药药理与毒理研究。
  • 基金资助:
    基金项目：国家973计划中医基础理论专项,确有疗效的有毒中药科学应用关键问题的基础研究（项目编号：2009CB522800）; 有毒中药的毒性与功效、证候关系的基础研究（课题编号：2009CB522802）; 国家中医药管理局中医药科学技术研究专项—“有毒中药”质量控制及有毒成分限量示范研究（国中医药科2004ZX00）

Experimental Study on Rats' Acute Toxicity of Herba leonuri and its Processed Products

HUANG Wei1,2, SUN Rong2,*, ZHANG Zuo-ping3   

  1. 1Shandong Research Academy of TCM, Shandong Jinan, 250014, China;
    2Shandong University of Traditional Chinese Medicine, Shandong Jinan, 250355,China;
    3Jinan Xinglin Biology Techonology Corporation, Shandong Jinan, 250101, China
  • Received:2016-02-19 Revised:2016-02-19 Online:2010-02-08 Published:2016-02-19

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摘要： 目的 比较鲜益母草、干益母草和酒炙益母草不同炮制品95%乙醇热回流提取物对小鼠的急性毒性大小,探讨益母草炮制减毒原理,优选炮制工艺。方法 分别制备鲜益母草、干益母草和酒炙益母草95%乙醇热回流提取物,按照经典急性毒性实验方法,进行益母草不同炮制品的急性毒性实验研究,实验数据用Bliss法计算LD50。结果 益母草不同炮制品对小鼠急性毒性强度以鲜益母草毒性最大,干益母草次之,酒炙益母草毒性最低。鲜益母草和干益母草95％乙醇热回流提取物LD50按含生药量计算分别为83.089 g·kg-1和102.93 g·kg-1,相当于每公斤人日用生药量的145.4倍和240.2倍;95%可信限分别为71.302~97.055 g·kg-1和91.862~115.140 g·kg-1,相当于每公斤人日用生药量的124.8~169.9倍和214.4~268.7倍。酒炙益母草95％乙醇热回流提取物无法作出LD50,MTD实验结果按含生药量计算为98.0g·kg-1,相当于临床70 kg人每公斤体重日用量的228.7倍。结论 炮制可降低益母草的毒性,不同炮制方法的减毒作用有差异,通过比较研究有利于实现饮片规范化、标准化,为临床用药提供安全标准和可控依据。

关键词: 益母草, 炮制品, 95%乙醇热回流提取物, 小鼠, 急性毒性

Abstract: Objective To approach the principle of the processing method and optimize the best processed products of herba leonuri, by comparing the acute toxicity of fresh herba leonuri、dry herba leonuri and wine herba leonuri. Methods Respectively prepare the 95% alcohol extracts of fresh herba leonuri、dry herba leonuri and wine herba leonuri, and make acute toxicity study according to classic acute toxicity Methods. The bliss method is used to calculate experimental datas' LD50. Results The different processed products' acute toxicity of herba leonuri from the biggest to the smallest is fresh herba leonuri、dry herba leonuri、wine herba leonuri. The fresh herba leonuri and dry herba leonuri extracts' LD50 is respectively 83.089g·kg-1 and 102.93 g·kg-1, which is respectively equal to 145.4 times and 2 40.2 times of every people's daily dried medicinal herb expenses; 95% confidence interval is respectively 71.302~97.055g·kg-1 and 91.862~115.140g·kg-1, which is respectively equal to 124.8~169.9 times and 214.4~268.7 times of every people's daily dried medicinal herb expenses. The wine herba leonuri extracts' MTD is 98.0g·kg-1, which is respectively equal to 228.7 times of every people's daily dried medicinal herb expenses. Conclusion The toxicity of herba leonuri could be decreased after processing, and the attenuation of different processed Methods is different. By the comparative study, it is beneficial to implement the normalization and standardization of cut crude drugs, and provide the safety standard and control basis for clinical medication.

Key words: Herba leonuri, Processed product, 95% alcohol extract, rats, acute toxicity

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